MONOCYTE CHEMOTAXIS - STIMULATION BY SPECIFIC EXOSITE REGION IN THROMBIN
MONOCYTE CHEMOTAXIS - STIMULATION BY SPECIFIC EXOSITE REGION IN THROMBIN
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DOI：
10.1126/science.6836310
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
FENTON, JW
中科院分区：
文献类型：
--
作者：
BARSHAVIT, R;KAHN, A;FENTON, JW
人类.alpha。凝血酶是一种有效的人单核细胞化学引诱剂，其最佳活性约为10 nmol/l。多种凝血酶经化学修饰以改变促凝血或溶脂功能，显示出类似的最佳活性，但凝血酶原或α的复合物。-凝血酶与抗凝血酶III或水蛭素均无明显差异。凝血酶中负责单核细胞趋化的区域显然与参与α蛋白某些蛋白质识别相互作用的区域接近。-凝血酶（例如水蛭素结合），但与催化位点和凝血所需的某些外源物不同。
Human .alpha.-thrombin is a potent chemoattractant for human monocytes, with optimum activity occurring at about 10 nmol/l. A variety of thrombins that were chemically modified to alter procoagulant or esterolytic functions showed a similar optimum activity, but complexes of prothrombin or .alpha.-thrombin with either antithrombin III or hirudin did not. The regions in thrombin responsible for monocyte chemotaxis apparently are proximate to those involved in certain protein recognition interactions of .alpha.-thrombin (e.g., hirudin binding) but are distinct from the catalytic site and from certain exosites required for clotting.