Structure and mass analysis of 12S and 19S dynein obtained from bull sperm flagella.
Structure and mass analysis of 12S and 19S dynein obtained from bull sperm flagella.
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从公牛精子鞭毛中获得的 12S 和 19S 动力蛋白的结构和质量分析。
DOI：
10.1002/cm.970080409
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发表时间：
1987
影响因子：
--
通讯作者：
Johnson,KA
中科院分区：
文献类型：
--
作者：
Marchese-Ragona,SP;Gagnon,C;White,D;Isles,MB;Johnson,KA
用Brookhaven扫描透射电子显微镜（STEM）研究了从公牛精子鞭毛中提取的12S和19S动力蛋白的结构和质量。12S粒子是一个球形粒子，平均质量为311 ± 10 kdalton。19S动力蛋白颗粒由两个球形头部连接到一个共同的基地。19 S粒子的平均质量为1.6 ± 0.04 × 106道尔顿。因此，除了较大的质量外，牛精子19S动力蛋白分子类似于从海胆精子鞭毛中获得的双头21S动力蛋白和从衣原体中获得的18S动力蛋白，可能有第三个头产生12S颗粒。公牛精子鞭毛动力蛋白的结构，质量和多肽组成进行了比较与外臂动力蛋白以前获得的衣原体，四膜虫，和海胆精子鞭毛。
The Brookhaven scanning transmission electron microscpe (STEM) was used to elucidate the structures and masses of 12S and 19S dynein extracted from bull sperm flagella. The 12S particle was a single globular particle with an average mass of 311 ± 10 kdaltons. The 19S dynein particles consisted of two globular heads joined to a common base. The average mass of the 19S particle was 1.6 ± 0.04 × 106daltons. Thus, with the exception of the larger mass, the bull sperm 19S dynein molecule resembles the two‐headed 21S dynein obtained from sea urchin sperm flagella and the 18S dynein obtained fromChlamydomonaswith the possibility of a third head giving rise to the 12S particle. The structure, mass and polypeptide composition of bull sperm flagella dynein is compared with outer arm dyneins previously obtained fromChlamydomonas,Tetrahymena, and sea urchin sperm flagella.