α-Complementation in an artificial genome replication system in liposomes
α-Complementation in an artificial genome replication system in liposomes
复制标题
脂质体人工基因组复制系统中的 α-互补
作者：
Nishiyama;K.;Ichihashi;N.;Matsuura;T.;Kazuta;Y.;Yomo;T
基因组大小被认为是原始生命形式复制的限制因素之一。然而，基因组大小和复制效率之间的关系尚未经过实验测试。在这项研究中，我们通过使用人工细胞模型（封装在脂质体中的自我复制 RNA 基因组）研究了基因组大小对基因组复制的影响。为了减小基因组大小，我们使用了 lacZ 基因的 α 互补。我们首先在纯化的翻译系统中表征了α-互补，然后将α-互补应用于基因组复制系统。基因组大小的减小加上 ω 片段的添加使复制效率提高了约八倍。这一结果提供了实验证据，表明基因组大小可能是原始自我复制系统的限制因素。这也意味着这种人工细胞模型可能是一个有用的实验模型，用于识别基因组扩增的可能机制。
Genome size is considered one of the limiting factors for the replication of primitive life forms. However, the relationship between genome size and replication efficiency has not been tested experimentally. In this study, we examined the effect of genome size on genome replication by using an artificial cell model: a self‐replicating RNA genome encapsulated in a liposome. For the reduced genome size we used α‐complementation of thelacZgene. We first characterized α‐complementation in the purified translation system and then applied α‐complementation to the genome replication system. The reduction in the genome size together with the addition of ω‐fragment increased the replication efficiency approximately eightfold. This result provides experimental evidence that genome size can be a limiting factor for primitive self‐replication systems; it also implies that this artificial cell model could be a useful experimental model to identify possible mechanisms of genome enlargement.