Microenvironment-triggered dual-activationof a photosensitizer fluorophore conjugate for tumor specific imaging and photodynamic therapy
Microenvironment-triggered dual-activationof a photosensitizer fluorophore conjugate for tumor specific imaging and photodynamic therapy
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微环境触发光敏剂荧光团缀合物的双重激活用于肿瘤特异性成像和光动力治疗
DOI：
10.1038/s41598-020-68847-w
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Xin Li
中科院分区：
文献类型：
--
作者：
Change Wang;Shengdan Wang;Yuan Wang;Honghai Wu;Kun Bao;Rong Sheng;Xin Li
光动力疗法正吸引越来越多的关注，但如何提高其肿瘤特异性仍然是一个艰巨的挑战。本文报道了一种结合焦脱镁叶绿酸α作为光敏剂和近红外荧光团用于肿瘤成像的治疗诊断探针（azo-pDt）。在正常条件下，光敏剂的光毒性和荧光团的荧光都受到抑制，而在缺氧条件下，偶氮基团的还原断裂将恢复这两种功能，导致肿瘤特异性荧光成像和光毒性。结果表明，azo-PDT选择性地对缺氧条件下的BEL-7402细胞成像，并对缺氧条件下近红外辐射后的BEL-7402细胞增殖具有抑制作用，而在常氧条件下对BEL-7402细胞的存活率几乎没有影响，这些结果证实了我们设计的提高光动力治疗肿瘤靶向能力的策略的可行性，偶氮pDt探针是一种很有前途的双功能试剂。
Photodynamictherapyisattractingincreasingattention,buthowtoincreaseitstumor‑specificity.remains a daunting challenge. Herein we report a theranostic probe (azo‑pDt) that integrates .pyropheophorbideαasaphotosensitizerandaNIRfluorophorefortumorimaging.Thetwo.functionalities are linked with a hypoxic‑sensitive azo group. Under normal conditions, both the .phototoxicityofthephotosensitizerandthefluorescenceofthefluorophoreareinhibited.While.under hypoxic condition, the reductive cleavage of the azo group will restore both functions, leading .totumorspecificfluorescenceimagingandphototoxicity.Theresultsshowedthatazo‑PDTselectively.imagesBEL‑7402cellsunderhypoxia,andsimultaneouslyinhibitsBEL‑7402cellproliferationafter.near‑infraredirradiationunderhypoxia,whilelittleeffectonBEL‑7402cellviabilitywasobserved.undernormoxia.Theseresultsconfirmthefeasibilityofourdesignstrategytoimprovethetumor‑.targeting ability of photodynamic therapy, and presents azo‑pDt probe as a promising dual functional .agent.