Synthesis of E7 peptide-modified biodegradable polyester with the improving affinity to mesenchymal stem cells
Synthesis of E7 peptide-modified biodegradable polyester with the improving affinity to mesenchymal stem cells
复制标题
E7肽修饰的可生物降解聚酯的合成，提高对间充质干细胞的亲和力
DOI：
10.1016/j.msec.2016.12.088
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发表时间：
2017-04-01
影响因子：
7.9
通讯作者：
Gao, Changyou
中科院分区：
文献类型：
--
作者：
Li, Qian;Xing, Dongming;Gao, Changyou
骨髓间充质干细胞(BMSCs)作为最具发展前景的干细胞，在再生医学领域得到了广泛的关注和应用。一种与骨髓间充质干细胞亲和力可调的可生物降解聚酯在决定骨髓间充质干细胞构建物的性能方面起着至关重要的作用。本研究通过丙交酯(LA)与呋喃-马来内酯功能化三亚甲基碳酸酯(FIVITMC)的开环共聚和Diels-Alder反应合成了马来酰亚胺功能化可生物降解聚酯(P(MTMC-LA))。用不同量的BMSCs特异性亲和肽(EPLQLKM、E7)修饰P(MTMC-LA)，通过点击化学方法研究其对BMSCs的影响。将E7多肽修饰的P(MTMC-LA)在玻片上浇铸成膜，并在膜上种植BMSCs。体外实验表明，与未修饰的P(MTMC-LA)膜相比，E7多肽修饰的P(MTIVIC-LA)膜可促进BMSCs的黏附和增殖。此外，随着多肽接枝率的增加，粘附性和增殖性增强。这些结果表明，新型可生物降解聚酯可以作为一种在组织工程和再生医学中具有巨大应用潜力的生物材料。(C)2016爱思唯尔B.V.保留所有权利。
As the most promising stem cell, bone marrow-derived mesenchymal stem cells (BMSCs) has attracted many attentions and applied widely in regenerative medicine. A biodegradable polyester with tunable affinity to BMSCs plays critical role in determining the properties of the BMSCs-based constructs. In this study, maleimide functionalized biodegradable polyester (P(MTMC-LA)) was synthesized through ring-opening copolymerization between c-lactide (LA) and furan-maleinlide functionalized trimethylene carbonate (FIVITMC) and a subsequent retro Diels-Alder reaction. P(MTMC-LA) was modified by different amounts of BMSCs specific affinity peptide (EPLQLKM, E7) through click-chemistry to investigate the effect on BMSCs. The E7 peptide modified P(MTMC-LA) was casted into films on glass slides and BMSCs were seeded onto the films. In vitro study showed that E7 peptide modified P(MTIVIC-LA) films supported BMSCs adhesion and proliferation compared to unmodified P(MTMC-LA) film. Besides, the adhesion and proliferation were enhanced by the increasing peptide grafting ratio. These results indicated that the novel biodegradable polyester can serve as a biomaterial with great potential application in tissue engineering and regenerative medicine. (C) 2016 Elsevier B.V. All rights reserved.