Maternal cell microchimerism in newborn tissues
Maternal cell microchimerism in newborn tissues
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DOI：
10.1067/mpd.2003.mpd0327
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发表时间：
2003-01-01
影响因子：
5.1
通讯作者：
Bianchi, DW
中科院分区：
文献类型：
--
作者：
Srivatsa, B;Srivatsa, S;Bianchi, DW
目的根据在新生儿脐带血中检测到母体细胞的报道，我们验证了母体细胞可以从循环中迁移到新生儿组织中的假设。研究设计我们研究了4例新生儿的尸检材料，这些新生儿从未接受过输血，并在出生后第一周死亡。研究对象诊断为21三体伴非免疫性水肿、46、XY、4q+伴多发性先天性异常、波特综合征、先天性鱼鳞病。用X和Y染色体特异性探针对石蜡包埋组织切片进行荧光原位杂交分析，包括肝脏、脾脏、胸腺、甲状腺和皮肤。结果4例雄性新生儿的多种组织类型中均检测到完整细胞核和两条X染色体信号所定义的雌性细胞。每张载玻片的雌性细胞数量从3到45不等。结论母细胞进入胎儿循环并能向胎儿和新生儿器官迁移。这对于脐带血移植和自身免疫性疾病的产后发展的潜在后果是重要的。
Objectives On the basis of reports of maternal cells being detected in-the umbilical cord blood of newborn infants, we tested the hypothesis that maternal cells can migrate out of the circulation into newborn tissues.Study design We studied autopsy material from 4 newborn infants who never received a blood transfusion and died during the first week of life. The study subjects' diagnoses were trisomy 21 with nonimmune hydrops, 46,XY, 4q+ with multiple congenital anomalies, Potter syndrome, and congenital ichthyosis. Fluorescence in situ hybridization analysis with X and Y chromosome-specific probes was performed on sections of paraffin-embedded tissue, including liver, spleen, thymus, thyroid, and skin.Results Female cells, as defined by the presence of intact nuclei with two X chromosome signals, were detected in multiple tissue types from all 4 male neonates. The number of female cells varied from 3 to 45 per slide.Conclusions Maternal cells enter the fetal circulation and are capable of migration to fetal and neonatal organs. This is of importance with regard to potential consequences of umbilical cord blood transplantation and postnatal development of autoimmune disease.