Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens
Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens
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DOI：
10.1634/stemcells.20-4-329
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发表时间：
2002-01-01
期刊：
影响因子：
5.2
通讯作者：
Andrews, PW
中科院分区：
文献类型：
--
作者：
Henderson, JK;Draper, JS;Andrews, PW
细胞表面抗原为鉴定细胞和分析细胞分化提供了宝贵的工具。特别是，在早期胚胎发育过程中受到发育调控的阶段特异的胚胎抗原被广泛用作监测小鼠和人类胚胎干细胞及其恶性对应的胚胎癌细胞(EC细胞)分化的标志物。然而，在人和小鼠的ES/EC细胞中，一些这样的标记的表达模式存在显著的差异，到目前为止，还不清楚这是否表明人和小鼠胚胎之间的显著差异，或者ES/EC细胞是否对应于每个物种早期胚胎中不同的细胞类型。我们现在发现，人类ES细胞的特征是表达细胞表面抗原SSEA3、SSEA4、TRA-1-60和TRA-1-81，而缺乏SSEA1，与小鼠胚胎的相应细胞相反，人类囊胚的内细胞团细胞表达类似的抗原谱。
Cell-surface antigens provide invaluable tools for the identification of cells and for the analysis of cell differentiation. In particular, stage-specific embryonic antigens that are developmentally regulated during early embryogenesis are widely used as markers to monitor the differentiation of both mouse and human embryonic stem (ES) cells and their malignant counterparts, embryonic carcinoma (EC) cells. However, there are notable differences in the expression patterns of some such markers between human and mouse ES/EC cells, and hitherto it has been unclear whether this indicates significant differences between human and mouse embryos, or whether ES/EC cells correspond to distinct cell types within the early embryos of each species. We now show that human ES cells are characterized by the expression of the cell-surface antigens, SSEA3, SSEA4, TRA-1-60, and TRA-1-81, and by the lack of SSEA1, and that inner cell mass cells of the human blastocyst express a similar antigen profile, in contrast to the corresponding cells of the mouse embryo.