In Vitro Synergistic Enhancement of Newcastle Disease Virus to 5-Fluorouracil Cytotoxicity against Tumor Cells.
In Vitro Synergistic Enhancement of Newcastle Disease Virus to 5-Fluorouracil Cytotoxicity against Tumor Cells.
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DOI：
10.3390/biomedicines4010003
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发表时间：
2016-01-29
期刊：
影响因子：
4.7
通讯作者：
Duiach A
中科院分区：
文献类型：
--
作者：
Al-Shammari AM;Salman MI;Saihood YD;Yaseen NY;Raed K;Shaker HK;Ahmed A;Khalid A;Duiach A
背景：化疗是目前世界范围内广泛应用的抗肿瘤治疗方法之一，但其毒副作用严重，疗效不理想。已经进行了许多尝试来增加其活性并降低其毒性。5-氟尿嘧啶（5-FU）仍然是一种广泛使用的化疗药物，特别是与其他化疗药物联合使用。联合治疗似乎是通过不同机制靶向肿瘤细胞的最佳选择。病毒治疗因其安全性和选择性而成为一种很有前途的抗癌药物。纽卡斯尔病病毒是安全的，它选择性地靶向肿瘤细胞。我们以前证明，纽卡斯尔病病毒（NDV）可用于增加其他化疗药物，并减少其毒性的一半的管理剂量和取代淘汰的化疗药物与纽卡斯尔病病毒，保持相同的抗肿瘤活性。方法：在目前的工作中，我们在不同的肿瘤细胞系上测试了这一假设。我们使用NDV的无毒力LaSota株与5-FU组合，并且我们测量了细胞毒性效应。我们使用Chou-Talalay分析评估了这种组合。结果如下：NDV与5-FU在低剂量下联合治疗不同的癌细胞时具有协同作用，对非癌细胞的作用非常轻微。总结：毒性、非致病性NDV-LaSota株与标准化疗剂5-FU的组合在体外对不同的肿瘤细胞具有协同作用，表明这种组合可能是治疗癌症的重要的新辅助疗法。
Background: Chemotherapy is one of the antitumor therapies used worldwide in spite of its serious side effects and unsatisfactory results. Many attempts have been made to increase its activity and reduce its toxicity. 5-Fluorouracil (5-FU) is still a widely-used chemotherapeutic agent, especially in combination with other chemotherapies. Combination therapy seems to be the best option for targeting tumor cells by different mechanisms. Virotherapy is a promising agent for fighting cancer because of its safety and selectivity. Newcastle disease virus is safe, and it selectively targets tumor cells. We previously demonstrated that Newcastle disease virus (NDV) could be used to augment other chemotherapeutic agents and reduce their toxicity by halving the administered dose and replacing the eliminated chemotherapeutic agents with the Newcastle disease virus; the same antitumor activity was maintained. Methods: In the current work, we tested this hypothesis on different tumor cell lines. We used the non-virulent LaSota strain of NDV in combination with 5-FU, and we measured the cytotoxicity effect. We evaluated this combination using Chou–Talalay analysis. Results: NDV was synergistic with 5-FU at low doses when used as a combination therapy on different cancer cells, and there were very mild effects on non-cancer cells. Conclusion: The combination of a virulent, non-pathogenic NDV–LaSota strain with a standard chemotherapeutic agent, 5-FU, has a synergistic effect on different tumor cells in vitro, suggesting this combination could be an important new adjuvant therapy for treating cancer.