Xylitol production from xylose mother liquor: a novel strategy that combines the use of recombinant Bacillus subtilis and Candida maltosa.
Xylitol production from xylose mother liquor: a novel strategy that combines the use of recombinant Bacillus subtilis and Candida maltosa.
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DOI：
10.1186/1475-2859-10-5
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发表时间：
2011-02-07
影响因子：
6.4
通讯作者：
Deng Z
中科院分区：
文献类型：
--
作者：
Cheng H;Wang B;Lv J;Jiang M;Lin S;Deng Z
木糖母液具有高浓度的木糖（35%-40%）以及其它糖如L-阿拉伯糖（10%-15%）、半乳糖（8%-10%）、葡萄糖（8%-10%）和其它次要糖。由于该母液的复杂性，不可能通过简单方法进一步分离木糖。在中国，2009年生产了超过50，000公吨的木糖母液，并且存在于低成本酒中的木糖等糖的管理是一个问题。我们设计了一种新的策略，其中枯草芽孢杆菌和麦芽糖假丝酵母相结合，并用于将该母液中的木糖转化为木糖醇，一种更高价值的产品。首先用酵母菌C对木糖母液进行脱毒。麦芽糖，以除去B的抑制剂糠醛和5-羟甲基糠醛（HMF）。枯草杆菌生长存在于母液中的葡萄糖也被该酵母耗尽，这是一个额外的优点，因为葡萄糖导致B中的碳分解代谢物抑制。枯草杆菌。这种解毒处理产生了不含乙醇的母液，并且C。麦芽糖细胞可以在后期再用作生物催化剂以将木糖还原成木糖醇。在第二步中，重组B.构建了木糖异构酶基因被破坏的枯草杆菌菌株。将脱毒的木糖母液用作重组B的培养基。枯草杆菌培养，这导致培养基的L-阿拉伯糖耗尽和木糖富集。在第三步中，木糖被C.麦芽糖细胞，并从该酵母转化培养基中获得结晶木糖醇。C.麦芽糖转化木糖富集培养基的木糖醇产量为4.25 g L-1·h-1，木糖醇比生产率为0.85 g木糖醇/g木糖。本研究开发了一种从木糖母液中分离纯化木糖并利用C.麦芽糖介导的木糖生物氢化。
Xylose mother liquor has high concentrations of xylose (35%-40%) as well as other sugars such as L-arabinose (10%-15%), galactose (8%-10%), glucose (8%-10%), and other minor sugars. Due to the complexity of this mother liquor, further isolation of xylose by simple method is not possible. In China, more than 50,000 metric tons of xylose mother liquor was produced in 2009, and the management of sugars like xylose that present in the low-cost liquor is a problem. We designed a novel strategy in which Bacillus subtilis and Candida maltosa were combined and used to convert xylose in this mother liquor to xylitol, a product of higher value. First, the xylose mother liquor was detoxified with the yeast C. maltosa to remove furfural and 5-hydromethylfurfural (HMF), which are inhibitors of B. subtilis growth. The glucose present in the mother liquor was also depleted by this yeast, which was an added advantage because glucose causes carbon catabolite repression in B. subtilis. This detoxification treatment resulted in an inhibitor-free mother liquor, and the C. maltosa cells could be reused as biocatalysts at a later stage to reduce xylose to xylitol. In the second step, a recombinant B. subtilis strain with a disrupted xylose isomerase gene was constructed. The detoxified xylose mother liquor was used as the medium for recombinant B. subtilis cultivation, and this led to L-arabinose depletion and xylose enrichment of the medium. In the third step, the xylose was further reduced to xylitol by C. maltosa cells, and crystallized xylitol was obtained from this yeast transformation medium. C. maltosa transformation of the xylose-enriched medium resulted in xylitol with 4.25 g L-1·h-1 volumetric productivity and 0.85 g xylitol/g xylose specific productivity. In this study, we developed a biological method for the purification of xylose from xylose mother liquor and subsequent preparation of xylitol by C. maltosa-mediated biohydrogenation of xylose.
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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