Interruption of pregnancy in the rabbit by the administration of estrogen.
Interruption of pregnancy in the rabbit by the administration of estrogen.
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通过给予雌激素来中断兔子的妊娠。
DOI：
10.1002/jez.1401350305
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发表时间：
1957
期刊：
影响因子：
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通讯作者：
Gilbert S. Greenwald
中科院分区：
文献类型：
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作者：
Gilbert S. Greenwald
材料与方法：选用98只成年雌性新西兰白色家兔，测定17 β苯甲酸雌二醇终止妊娠的最小有效剂量。根据表1所示的时间表，连续三天对动物进行肌内注射。在最后一次注射后2至8天进行剖腹术。记录每个卵巢中黄体的数量以及子宫内膜的分布、大小和外观。在第一次剖腹手术后1周至10天，当难以确定子宫鞘中是否含有活的或死的胚胎时，将兔子打开。将总共20个子宫标本固定在用二氧六环澄清的布因溶液中，石蜡包埋，以10 μ m切片，并用azan或苏木精和伊红染色。为了确定雌激素对tuba1卵周围蛋白（粘蛋白）沉积的影响，进行了以下程序：三只动物在交配后立即给予5 μ g雌二醇并在24小时后处死，7只动物在交配后第一天给予5 μ g雌二醇并在48小时处死，9只动物在交配后24和48小时注射雌二醇5 μ g，并在交配后72小时处死，9只动物在交配后96小时分别在交配后24、48和72小时注射雌二醇5 μ g。由于文中解释的原因，该剂量方案后来修改为在coitunz后立即给予5 pg雌二醇，48小时后给予5 pg雌二醇。在前4天内，每隔24小时处死共20只对照动物。
MATERIALS AND METHODSA total of 98 mature female New Zealand white rabbits were used to determine the minimum effective dose of 17p estradiol benzoate which would terminate pregnancy. The animals were injected intramuscularly for three consecutive days according to the schedule shown in table 1. Laparotomies were performed two to 8 days after the last injection. The number of corpora lutea in each ovary and the distribution, size, and appearance of uterine swellings was noted. The rabbit was opened a week to 10 days after the first laparotomp, whenever it was difficult to determine whether uterine swellings contained living or dead embryos. A total of 20 uterine specimens were fixed in Bouin's solution cleared in dioxane, embedded in paraffi, sectioned at 10 p, and stained with either azan or hematoxylin and eosin. In order to determine the effect of estrogen on the deposition of albumen (mucin) around tuba1 eggs, the following procedures were carried out: three animals were given 5 pg of estradiol immediately post coitzlm and killed 24 hours later, 7 animals were given 5 pg of estradiol at one day after mating and killed at 48 hours, 9 animals were injected with 5pg of estradiol at 24 and 48 hours after mating and killed 72 hours post coitum, 9 animals killed at 96 hours were given 5pg of estradiol at 24, 48, and 72 hours after mating. This dosage schedule was later modified to 5 pg of estradiol immediately post coitunz and 5 pg 48 hours later, for reasons explained in the text. A total of 20 control animals were killed at 24 hour intervals during the first 4 days.