Drastic change of local stiffness distribution correlating to cell migration in living fibroblasts
Drastic change of local stiffness distribution correlating to cell migration in living fibroblasts
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DOI：
10.1002/cm.10008
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发表时间：
2001-12-01
影响因子：
--
通讯作者：
Kawabata, K
中科院分区：
文献类型：
--
作者：
Nagayama, M;Haga, H;Kawabata, K
在力调制模式下，使用扫描探针显微镜捕获了培养条件下活成纤维细胞（NIH3T3）局部刚度分布的序列图像。我们发现细胞迁移与细胞局部刚度分布之间有明显的关系：当细胞在一个位置静止时，其细胞表面的刚度分布相当稳定。另一方面，一旦细胞开始移动，核区的硬度就会急剧下降。对细胞迁移和细胞刚度之间的关系提出了可能的解释。细胞Motil。中国生物医学工程学报，2009,31(2):393 - 398。(C) 2001 Wiley-Liss, Inc。
Sequential images of the local stiffness distribution of living fibroblasts (NIH3T3) were captured under a culture condition using scanning probe microscopy in a force modulation mode. We found a clear relation between cell mi-ration and local stiffness distribution on the cell: When cells were stationary at one position, the stiffness distribution of their cellular surface was quite stable. On the other hand, once the cells started to move, the stiffness in their nuclear regions drastically decreased. Possible explanations for the correlation between the cell mi-ration and the cell stiffness are proposed. Cell Motil. Cytoskeleton 50: 173-179,2001. (C) 2001 Wiley-Liss, Inc.