Size-dependent toxicity of silica nano-particles to Chlorella kessleri
Size-dependent toxicity of silica nano-particles to Chlorella kessleri
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DOI：
10.1080/10934520802171675
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Moritoki, Nobuko
中科院分区：
文献类型：
--
作者：
Fujiwara, Kitao;Suematsu, Hitoshi;Moritoki, Nobuko
发现SiO2纳米颗粒对小球藻（Chlorella kessleri）表现出尺寸依赖的毒性。小的SiO2纳米颗粒表现出更强的毒性：5 nm的50%抑制浓度（IC 50）值= 0.8 +/-0.6%，26 nm = 7.1 +/-2.8%，78 nm = 9.1 +/-4.7%。通过流式细胞术观察到细胞体的包围，这是由于存在阻碍细胞分裂的结构。用光学显微镜和透射显微镜观察分裂不完全的凝固细胞。虽然SiO2纳米颗粒的生理效应尚不清楚，但SiO2纳米颗粒至少对水生介质中的藻类是有毒的。在透射电子显微镜下，几个无定形结构出现在细胞暴露于5纳米二氧化硅纳米颗粒。
SiO2 nano-particles were found to exhibit size-dependent toxicity toward the alga, Chlorella kessleri. Small SiO2 nano-particles exhibit stronger toxicity: 50% inhibitory concentrations (IC50) value for 5 nm = 0.8 +/- 0.6%, 26 nm = 7.1 +/- 2.8%, and 78 nm = 9.1 +/- 4.7%. Enlargement of the cell body was observed by flow cytometry, which is due to the presence of structures that obstructed cell division. Optical and transmission microscopes were used to observe coagulated cells with incomplete division. Although the physiological effect of SiO2 nano-particles was not clear, SiO2 nano-particles are toxic, at least for algae in aquatic media. Under the transmission electron microscope, several amorphous structures appeared in the cells that were exposed to 5-nm silica nano-particles.