Somatic cell count and type of intramammary infection impacts fertility from in vitro produced embryo transfer
Somatic cell count and type of intramammary infection impacts fertility from in vitro produced embryo transfer
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DOI：
10.1016/j.theriogenology.2017.12.025
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发表时间：
2018-03-01
期刊：
影响因子：
2.8
通讯作者：
Vasconcelos, J. L. M.
中科院分区：
文献类型：
--
作者：
Barbosa, L. F. S. P.;Oliveira, W. V. C.;Vasconcelos, J. L. M.
本研究旨在评估乳腺炎致菌和体细胞计数（SCC）对荷尔斯坦-吉尔杂交（吉兰多）泌乳奶牛每胚胎移植妊娠（P/ET）的影响。奶牛（n = 1397）接受定时胚胎移植方案。在胚胎移植前两天采集乳汁样本进行SCC和细菌培养分析。定时胚胎移植后第31天和第66天进行妊娠诊断。动物根据国家乳腺炎委员会的建议分组：革兰氏阳性环境（EV+），革兰氏阴性环境（EV-），革兰氏阳性传染性（C+），凝固酶阴性葡萄球菌（CNS）和对照（无细菌生长）。根据致病性程度（主要或次要）对细菌进行分类。在妊娠第31天和第66天，细菌的生长降低了P/ET （P < 0.01）。第31天，EV-组（30.1%）和EV+组（29.9%）P/ET均低于对照组（44.0%）（P < 0.05），且C+组（36.6%）有低于对照组（P = 0.09）的趋势。妊娠31天，大剂量组的P/ET低于小剂量组和对照组（分别为32.1 vs 41.1 vs 43.2%） （P = 0.03）。妊娠第31天（30.4%比40.8%）和第66天（24.7%比32.2%），SCC浓度为40万细胞/mL的奶牛的P/ET均低于SCC浓度< 20万细胞/mL的奶牛（P < 0.01）。妊娠损失在细菌分离株和SCC类别之间没有差异。升高的SCC显著降低了P/ET，而环境因素和具有主要致病性的因素产生了最大的NET降低。(C) 2017爱思唯尔公司版权所有。
The objective of this study was to assess the impact of mastitis-causing bacteria and somatic cell count (SCC) on pregnancy per embryo transfer (P/ET) in Holstein-Gir crossbred (Girolando) lactating dairy cows. Cows (n = 1397) were subjected to a timed-embryo transfer protocol. Milk samples were collected two days before embryo transfer for SCC and bacteriological culture analyses. Pregnancy diagnosis was performed on days 31 and 66 after timed-embryo transfer. The animals were grouped according to the National Mastitis Council recommendations: Gram-positive environmental (EV+), Gram-negative environmental (EV-), Gram-positive contagious (C+), coagulase-negative staphylococci (CNS) and control (no bacterial growth). Additional analysis was made by categorizing bacteria based on degree of pathogenicity (Major or Minor). Bacterial growth reduced P/ET (P < .01) at both 31 and 66 days of gestation. The P/ET was lower (P < .05) at 31 days in EV- (30.1%) and EV+ (29.9%) groups and tended (P = .09) to be lower in the C+ group (36.6%) than the control group (44.0%). The P/ET from the Major group at 31 days of gestation was lower (P = .03) compared with the Minor and control groups (32.1 vs 41.1 vs 43.2%, respectively). Cows with SCC > 400,000 cells/mL had lower P/ET (P < .01) than animals with SCC < 200,000 cells/mL at both 31 (30.4% vs 40.8%) and 66 days (24.7% vs 32.2%) of gestation. Pregnancy loss was not different between bacterial isolates and SCC categories. Elevated SCC significantly reduced P/ET, whereas environmental agents and those with Major pathogenicity yielded the greatest reduction in NET. (C) 2017 Elsevier Inc. All rights reserved.