Utility of COI, CAD and morphological data for resolving relationships within the genus Sarcophaga (sensu lato) (Diptera: Sarcophagidae): a preliminary study.
Utility of COI, CAD and morphological data for resolving relationships within the genus Sarcophaga (sensu lato) (Diptera: Sarcophagidae): a preliminary study.
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DOI：
10.1016/j.ympev.2013.04.034
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发表时间：
2013-10
影响因子：
4.1
通讯作者：
K. Meiklejohn;J. Wallman;T. Pape;S. Cameron;M. Dowton
中科院分区：
文献类型：
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作者：
K. Meiklejohn;J. Wallman;T. Pape;S. Cameron;M. Dowton
目前已知的肉蝇科(∼)有3000种，隶属于3亚科173属。近25%的石棺化石属于石棺属(Sensu Lato)，但人们对石棺属∼150(或更多)亚属的有效性及其之间的关系知之甚少。在这项初步研究中，我们评估了三个数据来源在解决来自14个亚属35个物种之间的关系的有用性：线粒体COI条形码区域，核基因CAD800hp的∼，以及110个形态特征。树的大部分只得到贝叶斯和最大似然分析的支持，而MP树的解析很差。在贝叶斯分析和最大似然分析中，该属均被确定为单系种，并在种水平上得到了强有力的支持。值得注意的是，唯一一致被解析为单系的亚属是Liopygia。所采样的其余Sarcophaga s.l.亚属的单性及其之间的关系仍然值得怀疑。我们建议未来对Sarcophaga s.l.属的系统发育研究使用组合数据集进行分析。我们还主张使用额外的数据和一系列推理策略来帮助解决Sarcophaga s.l中的关系。
Currently there are ∼3000 known species of Sarcophagidae (Diptera), which are classified into 173 genera in three subfamilies. Almost 25% of sarcophagids belong to the genusSarcophaga(sensu lato) however little is known about the validity of, and relationships between the ∼150 (or more) subgenera ofSarcophaga s.l. In this preliminary study, we evaluated the usefulness of three sources of data for resolving relationships between 35 species from 14Sarcophaga s.l.subgenera: the mitochondrial COI barcode region, ∼800 bp of the nuclear gene CAD, and 110 morphological characters.Bayesian, maximum likelihood (ML) and maximum parsimony (MP) analyses were performed on the combined dataset. Much of the tree was only supported by the Bayesian and ML analyses, with the MP tree poorly resolved. The genusSarcophaga s.l.was resolved as monophyletic in both the Bayesian and ML analyses and strong support was obtained at the species-level. Notably, the only subgenus consistently resolved as monophyletic wasLiopygia. The monophyly of and relationships between the remainingSarcophaga s.l.subgenera sampled remain questionable. We suggest that future phylogenetic studies on the genusSarcophaga s.l.use combined datasets for analyses. We also advocate the use of additional data and a range of inference strategies to assist with resolving relationships withinSarcophaga s.l.