Effects of tissue processing on bioactivity of cartilage matrix-based hydrogels encapsulating osteoconductive particles.
Effects of tissue processing on bioactivity of cartilage matrix-based hydrogels encapsulating osteoconductive particles.
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DOI：
10.1088/1748-605x/aaad77
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发表时间：
2018-03-16
期刊：
影响因子：
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通讯作者：
Detamore MS
中科院分区：
文献类型：
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作者：
Townsend JM;Zabel TA;Feng Y;Wang J;Andrews BT;Nudo RJ;Berkland CJ;Detamore MS
在严重创伤性脑损伤（TBI）的治疗中，去骨瓣减压术通常用于去除大部分颅骨，以使脑肿胀不受阻碍。水凝胶有可能通过允许单次手术干预来彻底改变TBI治疗，在脑肿胀期间保持柔韧性，并在肿胀消退后进行调整以再生骨。出于这个动机，我们的目标是提出一种柔韧的材料，能够在关键尺寸的缺损处再生颅骨。因此，我们提出了使用甲基丙烯酸酯化溶解脱细胞软骨（MeSDCC）水凝胶包封羟基磷灰石纳米纤维（HAPnf），玻璃微粒（BG），或添加大鼠骨髓来源的间充质干细胞（rMSCs）的合成成骨颗粒在临界大小的大鼠颅骨缺损的骨再生。使用纤维素水凝胶作为研究的对照材料。MeSDCC水凝胶在交联之前表现出足够的流变性能用于材料放置（τy > 500 Pa），并且在交联后表现出足够的压缩模量（E > 150 kPa）。体外实验表明，MeSDCC材料上接种的细胞钙沉积增加;然而，即使使用胶体材料或添加的rMSC，MeSDCC和纤维蛋白组的体内骨再生也是最小的。MeSDCC试验组中最小的骨再生可能潜在地归因于脱细胞化后的软骨溶解，其中材料信号可能已从酶处理中降解。展望未来，材料生物活性的改善将是TBI治疗骨再生策略成功的关键。
In the treatment of severe traumatic brain injury (TBI), decompressive craniectomy is commonly used to remove a large portion of calvarial bone to allow unimpeded brain swelling. Hydrogels have the potential to revolutionize TBI treatment by permitting a single-surgical intervention, remaining pliable during brain swelling, and tuned to regenerate bone after swelling has subsided. With this motivation, our goal is to present a pliable material capable of regenerating calvarial bone across a critical size defect. We therefore proposed the use of a methacrylated solubilized decellularized cartilage (MeSDCC) hydrogel encapsulating synthetic osteogenic particles of hydroxyapatite nanofibers (HAPnf), bioglass microparticles (BG), or added rat bone marrow-derived mesenchymal stem cells (rMSCs) for bone regeneration in critical-size rat calvarial defects. Fibrin hydrogels were employed as a control material for the study. MeSDCC hydrogels exhibited sufficient rheological performance for material placement before crosslinking (τy > 500 Pa), and sufficient compressive moduli post-crosslinking (E > 150 kPa). In vitro experiments suggested increased calcium deposition for cells seeded on the MeSDCC material; however, in vivo bone regeneration was minimal in both MeSDCC and fibrin groups, even with colloidal materials or added rMSCs. Minimal bone regeneration in the MeSDCC test groups may potentially be attributed to cartilage solubilization after decellularization, in which material signals may have degraded from enzymatic treatment. Looking to the future, an improvement in the bioactivity of the material will be crucial to the success of bone regeneration strategies for TBI treatment.