Ammonium supply rate influences archaeal and bacterial ammonia oxidizers in a wetland soil vertical profile
Ammonium supply rate influences archaeal and bacterial ammonia oxidizers in a wetland soil vertical profile
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DOI：
10.1111/j.1574-6941.2010.00961.x
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发表时间：
2010-11-01
影响因子：
4.2
通讯作者：
Mandic-Mulec, Ines
中科院分区：
文献类型：
--
作者：
Hoefferle, Spela;Nicol, Graeme W.;Mandic-Mulec, Ines
氨氧化是硝化作用的第一步，在土壤中由细菌和古细菌氨氧化菌进行，最近的研究表明，在低铵环境中可能选择后者。在这项研究中，我们调查了氨氧化古生菌和细菌在湿地土壤垂直剖面中的选择，这两个地点的氨供应速率不同，但地下水位的差异不显著。一个站点通过分解有机物获得铵，而第二个受污染的站点通过地下化粪池的持续泄漏获得更多的供应。污染区土壤硝化潜力明显大于污染区。对amoA基因的定量研究表明，在污染场地的土壤剖面中，细菌amoA基因的丰度高于古生菌amoa基因，而在非污染场地细菌amoa基因的丰度低于检测下限。在这两个地点，古生菌，但不是细菌群落结构，在地下水位施加的土壤氧化还原潜力方面，随着深度明显分层。然而，古细菌群落结构的深度相关变化也可能与氨氧化以外的生理功能有关。
Oxidation of ammonia, the first step in nitrification, is carried out in soil by bacterial and archaeal ammonia oxidizers and recent studies suggest possible selection for the latter in low-ammonium environments. In this study, we investigated the selection of ammonia-oxidizing archaea and bacteria in wetland soil vertical profiles at two sites differing in terms of the ammonium supply rate, but not significantly in terms of the groundwater level. One site received ammonium through decomposition of organic matter, while the second, polluted site received a greater supply, through constant leakage of an underground septic tank. Soil nitrification potential was significantly greater at the polluted site. Quantification of amoA genes demonstrated greater abundance of bacterial than archaeal amoA genes throughout the soil profile at the polluted site, whereas bacterial amoA genes at the unpolluted site were below the detection limit. At both sites, archaeal, but not the bacterial community structure was clearly stratified with depth, with regard to the soil redox potential imposed by groundwater level. However, depth-related changes in the archaeal community structure may also be associated with physiological functions other than ammonia oxidation.