Lethal and Dermonecrotic Activities of Clostridium perfringens Iota Toxin: Biological Activities Induced by Cooperation of Two Nonlinked Components
Lethal and Dermonecrotic Activities of Clostridium perfringens Iota Toxin: Biological Activities Induced by Cooperation of Two Nonlinked Components
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产气荚膜梭菌 Iota 毒素的致死性和皮肤坏死活性：两种非连接成分协同作用诱导的生物活性
DOI：
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发表时间：
1995
影响因子：
2.6
通讯作者：
Keiko Kobayashi
中科院分区：
文献类型：
--
作者：
J. Sakùrai;Keiko Kobayashi
研究了单独注射产气荚膜梭菌iota毒素的两种组分（指定为Ia和Ib组分）对毒素生物活性的影响。在注射另一种组分后120 min内静脉注射一种组分可杀死小鼠。抗Ia或抗Ib抗血清可消除iota毒素的活性。另一方面，在静脉注射Ia成分后120分钟内，对给予抗Ia抗血清的小鼠静脉注射Ib成分时，致死活性被完全中和，但在注射Ib成分后5分钟以上，对给予抗Ib抗血清的小鼠静脉注射Ia成分时，活性未被中和。将Ia和Ib组分分别注射到豚鼠皮肤中，表明Ib组分注射部位出现皮肤坏死，但Ia组分注射部位未出现皮肤坏死。此外，当将一种组分皮内注射豚鼠，然后将另一种组分腹膜内注射时，在Ib组分的皮内注射部位观察到毒素的皮肤坏死活性，但在Ia组分的皮内注射部位未观察到毒素的皮肤坏死活性。从数据来看，iota毒素的致死和皮肤坏死活性似乎是通过Ib组分与组织上的特定位点结合而启动的。
The effect of separate injections of two components of Clostridium perfringens iota toxin, designated Ia and Ib components, on the biological activities of the toxin was investigated. The intravenous injection of one component within 120 min after the injection of the other component killed mice. The activity of iota toxin was abolished by anti‐Ia or anti‐Ib antiserum. On the other hand, when Ib component was intravenously administered to mice given anti‐Ia antiserum within 120 min after the intravenous injection of Ia component, the lethal activity was completely neutralized, but when Ia component was injected into mice that were given anti‐Ib antiserum over 5 min after the injection of Ib component, the activity was not neutralized. The separate injections of Ia and Ib components in skin of guinea pigs indicated dermonecrosis at the injection site of Ib components, but not at the site of Ia components. Furthermore, when one component was intradermally injected in guinea pigs and then the other intraperitoneally, the dermonecrotic activity of the toxin was observed at the intradermal injection site of Ib component, but not at that of Ia component. From the data, it appears that the lethal and dermonecrotic activities of iota toxin are initiated by the binding of Ib component to specific sites on tissues.
产气荚膜梭菌 iota 毒素：两种蛋白质之间的协同作用。
DOI：
10.1016/0041-0101(86)90101-7
发表时间：
1986
期刊：
Toxicon : official journal of the International Society on Toxinology
影响因子：
--
作者：
Stiles,BG;Wilkins,TD
通讯作者：
Wilkins,TD