Dexmedetomidine Pretreatment Attenuates Kidney Injury and Oxidative Stress during Orthotopic Autologous Liver Transplantation in Rats.
Dexmedetomidine Pretreatment Attenuates Kidney Injury and Oxidative Stress during Orthotopic Autologous Liver Transplantation in Rats.
复制标题
右美托咪定预处理减轻大鼠原位自体肝移植期间的肾损伤和氧化应激
DOI：
10.1155/2016/4675817
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发表时间：
2016
影响因子：
--
通讯作者：
Cai J
中科院分区：
文献类型：
--
作者：
Yu X;Chi X;Wu S;Jin Y;Yao H;Wang Y;Xia Z;Cai J
本研究旨在探讨右美托咪定（Dex）预处理在原位自体肝移植（OALT）过程中是否具有抗氧化和肾脏保护作用，以及其对核因子E2相关因子2（Nrf2）激活的影响。将Sprague - Dawley大鼠随机分为假手术组（S组）、模型组（M组）、低剂量Dex组（D1组）、高剂量Dex组（D2组）、阿替美唑（一种非特异性α2受体阻滞剂）+高剂量Dex组（B1组）、ARC239（一种特异性α2B/c受体阻滞剂）+高剂量Dex组（B2组）以及BRL - 44408（一种特异性α2A受体阻滞剂）+高剂量Dex组（B3组）。然后在OALT术后8小时对肾脏进行组织病理学检查，测量肾功能、肾脏Nrf2蛋白表达以及氧化剂和抗氧化剂水平。我们发现Dex预处理可激活肾小球细胞中的Nrf2，上调抗氧化剂水平，同时降低氧化剂水平（与M组相比，D2组所有指标均P < 0.01）。阿替美唑和BRL - 44408可逆转这些保护作用，但ARC239则不能。总之，Dex预处理通过α2A受体激活Nrf2，提高抗氧化剂水平，并减轻OALT过程中的肾脏损伤。
This paper aims to explore whether pretreatment with dexmedetomidine (Dex) has antioxidative and renal protective effects during orthotopic autologous liver transplantation (OALT) and its impact on nuclear factor erythroid 2-related factor 2 (Nrf2) activation. Sprague-Dawley rats were randomized into groups that include sham-operated (group S), model (group M), low dose Dex (group D1), high dose Dex (group D2), atipamezole (a nonspecific α 2 receptor blocker) + high dose Dex (group B1), ARC239 (a specific α 2B/c receptor blocker) + high dose Dex (group B2), and BRL-44408 (a specific α 2A receptor blocker) + high dose Dex (group B3). Then histopathologic examination of the kidneys and measurement of renal function, the renal Nrf2 protein expression, and oxidants and antioxidants were performed 8 hours after OALT. We found that pretreatment with Dex activated Nrf2 in glomerular cells and upregulated antioxidants but reduced oxidants (all P < 0.01, group D2 versus group M). Atipamezole and BRL-44408, but not ARC239, reversed these protective effects. In conclusion, pretreatment with Dex activates Nrf2 through α 2A receptor, increases the antioxidant levels, and attenuates renal injury during OALT.