Quantitative particle-induced X-ray emission imaging of rat olfactory epithelium applied to the permeability of rat epithelium to inhaled aluminum
Quantitative particle-induced X-ray emission imaging of rat olfactory epithelium applied to the permeability of rat epithelium to inhaled aluminum
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DOI：
10.1021/tx9900268
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发表时间：
1999-07-01
影响因子：
4.1
通讯作者：
Bench, G
中科院分区：
文献类型：
--
作者：
Divine, KK;Lewis, JL;Bench, G
慢性金属吸入的神经毒性被认为是晚期神经退行性疾病的潜在因素，这些疾病的症状与阿尔茨海默氏症和帕金森氏综合征相似。如果吸入的金属有助于这些疾病的发病机制，识别，定位和定量金属沉积（S）在中枢神经系统的特定目标区域将是至关重要的，我们的理解的机制。迄今为止使用的标准分析技术需要暴露于极高浓度的金属，以满足小组织区域的分析检测限。因此，与低剂量环境相关照射和潜在保护屏障的相关性值得怀疑。微束粒子诱导的X射线发射的可行性进行了研究，作为一种方法，用于快速扫描组织，研究吸入的金属，鼻渗透性，和中枢神经系统的沉积。确定了用于对大鼠嗅上皮进行成像以便于快速检测铝定位的最佳光束点和分析时间。铝定位在大鼠嗅球和脑切片的测量。
Neurotoxicity from chronic metal inhalation has been suggested as an underlying contributor to late-developing neurodegenerative diseases that have symptoms similar to Alzheimer's and Parkinson's syndromes. If inhaled metals contribute to pathogenesis of these diseases, identifying, localizing, and quantitating metal deposition(s) within specific target regions of the central nervous system will be critical to our understanding of the mechanisms. Standard analytical techniques used to date require exposure to extremely high concentrations of metals to meet analytical detection limits in small tissue areas. The relevance to lower-dose environmentally relevant exposures and potential protective barriers is therefore questionable. The feasibility of microbeam particle-induced X-ray emission is investigated as a method for rapidly scanning tissues to study the inhalation of metals, nasal permeability, and central nervous system deposition. The optimal beam spot and analysis time used to image the rat olfactory epithelium to facilitate the rapid detection of aluminum localizations were determined. Measurements of aluminum localizations in rat olfactory bulb and brain sections are also presented.