ISSR variations in eggplant (Solanum melongena L.) and related Solanum species
ISSR variations in eggplant (Solanum melongena L.) and related Solanum species
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DOI：
10.1016/j.scienta.2008.04.003
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发表时间：
2008-07-23
影响因子：
4.3
通讯作者：
Khan, Md. Mizanur Rahim
中科院分区：
文献类型：
--
作者：
Isshiki, Shiro;Iwata, Naoko;Khan, Md. Mizanur Rahim
为评价该方法在茄子系统发育关系和品种鉴定中的适用性，对8个茄子品种（Solanum melongena）和8个茄属近缘种的12份材料进行了ISSR分析。在100个引物中，34个引物共扩增出552条多态性条带，多态性比率为99.1%。基于ISSR标记的聚类分析将茄属植物分为7个类群：（i）S. melongena;（ii）S. aethiopicum和S. anguivi;（iii）S. incanum;（iv）S. violaceum和S. kurzii;（v）S. macrocarpon;（vi）S. virginianum和（vii）S. torvum。将34个引物中的少数引物扩增出的ISSR标记组合起来，即可区分8个茄子品种。证明该ISSR分析可用于系统发育研究和品种鉴定。(C)2008 Elsevier B. V.保留所有权利。
Inter-simple sequence repeat (ISSR) analysis was performed in eight cultivars of eggplant (Solanum melongena) and 12 accessions in eight related Solanum species to evaluate the applicability of this analysis for assessing the phylogenetic relationships and identifying cultivars. A total of 552 polymorphic amplified bands were obtained from 34 of the 100 primers tested, and the percentage of polymorphisms was 99.1%. Cluster analysis based on the ISSR markers classified the Solanum species into seven groups: (i) S. melongena; (ii) S. aethiopicum and S. anguivi; (iii) S. incanum; (iv) S. violaceum and S. kurzii; (v) S. macrocarpon; (vi) S. virginianum and (vii) S. torvum. Combining the ISSR markers obtained by a few of the 34 primers was enough for distinguishing of the eight cultivars of eggplant. This ISSR analysis was demonstrated to be available for the phylogenetic study and the cultivar identification. (C) 2008 Elsevier B.V. All rights reserved.