Microbial population structures in the deep marine biosphere
Microbial population structures in the deep marine biosphere
复制标题
DOI：
10.1126/science.1146689
复制
发表时间：
2007-10-05
期刊：
影响因子：
56.9
通讯作者：
Sogin, Mitchell L.
中科院分区：
文献类型：
--
作者：
Huber, Julie A.;Mark Welch, David;Sogin, Mitchell L.
环境DNA序列对微生物生态系统建模的分析能力取决于对种群结构的准确评估，包括多样性(丰富度)和相对丰度(均衡度)。我们通过检查90多万个微生物小亚基核糖体RNA扩增片段的序列，研究了两个相邻热液喷口微生物群落的种群结构的这两个方面。两个喷口群落具有不同的种群结构，反映了当地的地球化学制度。对古生物多样性的描述几乎是详尽的，但尽管收集了数量空前的序列，统计分析表明在每个分类水平上都有额外的细菌多样性。我们预测，捕捉微生物群落的巨大多样性将需要数十万个序列，高丰度和低丰度分类群的不同均匀模式可能对定义微生物生态系统动力学很重要。
The analytical power of environmental DNA sequences for modeling microbial ecosystems depends on accurate assessments of population structure, including diversity (richness) and relative abundance (evenness). We investigated both aspects of population structure for microbial communities at two neighboring hydrothermal vents by examining the sequences of more than 900,000 microbial small-subunit ribosomal RNA amplicons. The two vent communities have different population structures that reflect local geochemical regimes. Descriptions of archaeal diversity were nearly exhaustive, but despite collecting an unparalleled number of sequences, statistical analyses indicated additional bacterial diversity at every taxonomic level. We predict that hundreds of thousands of sequences will be necessary to capture the vast diversity of microbial communities, and that different patterns of evenness for both high- and low-abundance taxa may be important in defining microbial ecosystem dynamics.