Lymphocyte proliferative response to P6 of Haemophilus influenzae is associated with relative protection from exacerbations of chronic obstructive pulmonary disease
Lymphocyte proliferative response to P6 of Haemophilus influenzae is associated with relative protection from exacerbations of chronic obstructive pulmonary disease
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DOI：
10.1164/ajrccm.165.7.2109009
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发表时间：
2002-04-01
影响因子：
24.7
通讯作者：
Thanavala, YM
中科院分区：
文献类型：
--
作者：
Abe, Y;Murphy, TF;Thanavala, YM
慢性阻塞性肺疾病（COPD）的特征是周期性加重，其中一些是由不可分型的流感嗜血杆菌（NTHI）引起的。P6是NTHI菌株中高度保守的外膜脂蛋白。我们假设来自因NTHI而恶化的COPD患者的淋巴细胞识别P6的能力降低。本文研究了36例COPD患者和12例健康对照者对P6的体外淋巴细胞增殖反应。10名在过去12个月内因NTHI而加重的患者显示出统计学显著性较低的P6增殖（刺激指数，对数转换平均值+/-标准误0.82 +/- 0.17），与26例在过去12个月内未因NTHI而加重的患者相比（1.42 +/- 0.13）和12名健康对照受试者（1.61 +/- 0.16）。三组淋巴细胞对破伤风类毒素的增殖反应无明显差异。两组COPD患者血清抗P6抗体水平无差异。这些结果表明T细胞向P6的增殖减少与COPD的恶化相关，并表明T细胞识别P6的能力与对NTHI所致恶化的相对保护相关。
Chronic obstructive pulmonary disease (COPD) is characterized by periodic exacerbations, some of which are caused by nontypeable Haemophilus influenzae (NTHI). P6 is an outer membrane lipoprotein that is highly conserved among strains of NTHI We hypothesized that lymphocytes from patients with COPD who have exacerbations due to NTHI have a decreased ability to recognize P6. The in vitro lymphocyte proliferative response to P6 in 36 patients with COPD and 12 healthy control subjects was studied. Ten patients who had exacerbations due to NTHI in the previous 12 months showed statistically significant lower proliferation to P6 (stimulation index, log transformed mean +/- standard error 0.82 +/- 0.17) compared with 26 patients who had no exacerbations due to NTHI in the previous 12 months (1.42 +/- 0.13) and to 12 healthy control subjects (1.61 +/- 0.16). These three groups had no significant difference in the lymphocyte proliferative response to tetanus toxoid. There was no difference in serum antibody levels to P6 in the two groups with COPD. These results indicate that decreased proliferation of T cells to P6 is associated with exacerbations of COPD and suggest that the ability of T cells to recognize P6 is associated with relative protection from exacerbations due to NTHI.