Glucose modulates proliferation in root apical meristems via TOR in maize during germination
Glucose modulates proliferation in root apical meristems via TOR in maize during germination
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DOI：
10.1016/j.plaphy.2020.07.041
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发表时间：
2020-10-01
影响因子：
6.5
通讯作者：
Lara-Nunez, Aurora
中科院分区：
文献类型：
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作者：
Hugo Diaz-Granados, Victor;Manuel Lopez-Lopez, Jorge;Lara-Nunez, Aurora
葡萄糖靶向雷帕霉素（Glc-TOR）信号通路在不同的生物系统中已被研究，但在早期种子萌发过程中的研究较少。这项工作研究了其重要性的细胞增殖，细胞周期的关键基因的表达，其蛋白质水平，除了形态和细胞化的根顶端分生组织的玉米（玉米）胚轴在萌发过程中的影响下的两个简单的糖，葡萄糖和蔗糖，和TOR活性的特异性抑制剂，AZD 8055。这两种糖促进发芽的作用相似，并且在一定程度上与TOR活性无关。然而，Glc-TOR途径增加了致力于增殖的细胞数量，增加了细胞周期基因ZmCycD 4;2的表达，ZmCycD 4;2是一种推定的G1/S调节因子。此外，Glc-TOR可能对另一种G1/S细胞周期蛋白ZmCycD 3的蛋白质稳定性有影响，但对ZmCDKA;1或ZmKRP 3或其蛋白质没有影响。结果表明，Glc-TOR途径通过不同的机制参与增殖的调节，最终改变种子萌发的时间。
The Glucose-Target of Rapamycin (Glc-TOR) pathway has been studied in different biological systems, but scarcely during early seed germination. This work examines its importance for cell proliferation, expression of cell cycle key genes, their protein levels, besides morphology and cellularization of the root apical meristem of maize (Zea mays) embryo axes during germination under the influence of two simple sugars, glucose and sucrose, and a specific inhibitor of TOR activity, AZD 8055. The two sugars promote germination similarly and to an extent, independently of TOR activity. However, the Glc-TOR pathway increases the number of cells committed to proliferation, increasing the expression of a cell cycle gene, ZmCycD4;2, a putative G1/S regulator. Also, Glc-TOR may have influence on the protein stability of another G1/S cyclin, ZmCycD3, but had no influence on ZmCDKA;1 or ZmKRP3 or their proteins. Results suggest that the Glc-TOR pathway participates in the regulation of proliferation through different mechanisms that, in the end, modify the timing of seed germination.