Distinct subcellular localization of tRNA-derived fragments in the infective metacyclic forms of Trypanosoma cruzi
Distinct subcellular localization of tRNA-derived fragments in the infective metacyclic forms of Trypanosoma cruzi
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DOI：
10.1590/s0074-02762012000600018
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发表时间：
2012-09-01
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影响因子：
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通讯作者：
Dallagiovanna, Bruno
中科院分区：
文献类型：
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作者：
Reifur, Larissa;Garcia-Silva, Maria Rosa;Dallagiovanna, Bruno
来自转移RNA的小的非编码RNA已被鉴定为广泛保守的原核和真核细胞对应激的反应。它们的存在与基因表达调节相关的发育状态变化相一致。在克氏锥虫的外鞭毛体形式中，tRNA片段定位于后部细胞质颗粒。在寄生虫的感染性亚循环形式中，我们发现tRNA衍生的片段是丰富的，均匀分布在细胞质中。这些片段与多聚核糖体无关，表明tRNA衍生的片段可能不直接参与代谢环的翻译控制。
Small non-coding RNAs derived from transfer RNAs have been identified as a broadly conserved prokaryotic and eukaryotic response to stress. Their presence coincides with changes in developmental state associated with gene expression regulation. In the epimastigote form of Trypanosoma cruzi, tRNA fragments localize to posterior cytoplasmic granules. In the infective metacyclic form of the parasite, we found tRNA-derived fragments to be abundant and evenly distributed within the cytoplasm. The fragments were not associated with polysomes, suggesting that the tRNA-derived fragments may not be directly involved in translation control in metacyclics.