Temporal correlation of elevated PRMT1 gene expression with mushroom body neurogenesis during bumblebee brain development
Temporal correlation of elevated PRMT1 gene expression with mushroom body neurogenesis during bumblebee brain development
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DOI：
10.1101/449116
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发表时间：
2018-10
期刊：
影响因子：
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通讯作者：
Cui Guan;M. Egertová;Clint J. Perry;L. Chittka;A. Chittka
中科院分区：
文献类型：
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作者：
Cui Guan;M. Egertová;Clint J. Perry;L. Chittka;A. Chittka
昆虫神经的正常发育依赖于神经前体的可控增殖和分化。在全变态昆虫中，这些过程必须在幼虫和蛹的发育过程中协调。近年来，蛋白质精氨酸甲基化作为控制哺乳动物神经干细胞增殖和分化的重要机制而备受关注。类似的机制是否在昆虫身上起作用尚不清楚。我们通过原位杂交检测了三种蛋白质精氨酸甲基转移酶mRNAs(PRMT1、4和5)在熊蜂发育过程中大脑的表达模式，以探讨这种可能性。在整个发育过程中，我们检测到了神经前体和重要脑区神经元的表达。我们发现，在蘑菇体中含有分裂细胞的区域中，PRMT1的表达明显较高，而PRMT4和PRMT5在该脑区神经发生活跃的时期含有分裂细胞。在发育的后期，PRMT1的表达水平被发现是一致的，并且与活跃的细胞分裂无关。我们的研究表明，PRMT1在大黄蜂神经发生期间调节蘑菇体中的神经前体分裂方面发挥了作用。
Proper neural development in insects depends on the controlled proliferation and differentiation of neural precursors. In holometabolous insects, these processes must be coordinated during larval and pupal development. Recently, protein arginine methylation has come into focus as an important mechanism of controlling neural stem cell proliferation and differentiation in mammals. Whether a similar mechanism is at work in insects is unknown. We investigated this possibility by determining the expression pattern of three protein arginine methyltransferase mRNAs (PRMT1, 4 and 5) in the developing brain of bumblebees by in situ hybridisation. We detected expression in neural precursors and neurons in functionally important brain areas throughout development. We found markedly higher expression of PRMT1, but not PRMT4 and PRMT5, in regions of mushroom bodies containing dividing cells during pupal stages at the time of active neurogenesis within this brain area. At later stages of development, PRMT1 expression levels were found to be uniform and did not correlate with actively dividing cells. Our study suggests a role for PRMT1 in regulating neural precursor divisions in the mushroom bodies of bumblebees during the period of neurogenesis.