DNA-FERROUS IRON CATALYZED HYDROXYL FREE-RADICAL FORMATION FROM HYDROGEN-PEROXIDE
DNA-FERROUS IRON CATALYZED HYDROXYL FREE-RADICAL FORMATION FROM HYDROGEN-PEROXIDE
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DOI：
10.1016/0006-291x(81)90748-8
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
FLOYD, RA
中科院分区：
文献类型：
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作者：
FLOYD, RA
DNA以一种方式与Fe(II)结合，与在没有DNA的情况下形成的量相比，从H_2O_2生成的OH被增强。用自旋捕捉法测定羟基自由基的生成。最大。**图形**。当每个核苷酸的添加铁(II)的比例约为1/70时，形成。**图形**。用硫代巴比妥酸法测定DNA-Fe(II)络合物攻击DNA生成醛所生成的过氧化氢。这些数据暗示。**图形**引起的DNA链断裂。进攻。
DNA binds Fe(II) in a manner such that OH formation from H2O2 is enhanced as compared to the amount formed in the absence of DNA. Hydroxyl free radical formation was assayed using the spin-trap DMPO (5,5-dimethyl pyrroline-l-oxide). The maximum .**GRAPHIC**. formation occurs at an added Fe(II) per nucleotide ratio of about 1/70. The .**GRAPHIC**. formed H2O2 by the DNA-Fe(II) complex attacked DNA producing aldehydes as determined by the thiobarbituric acid method. These data implicate DNA strand breaks induced by .**GRAPHIC**. attack.