Cellular effects of industrial metal nanoparticles and hydrophilic carbon black dispersion
Cellular effects of industrial metal nanoparticles and hydrophilic carbon black dispersion
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DOI：
10.2131/jts.39.897
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发表时间：
2014-12-01
影响因子：
2
通讯作者：
Iwahashi, Hitoshi
中科院分区：
文献类型：
--
作者：
Horie, Masanori;Kato, Haruhisa;Iwahashi, Hitoshi
研究了金 (Au)、银 (Ag)、铂 (Pt)、Au-聚乙烯吡咯烷酮 (PVP) 胶体和 Pt-PVP 胶体五种金属纳米颗粒以及两种亲水性炭黑对细胞行为的影响。制备稳定的纳米颗粒分散体，并将其应用于人角质形成细胞 (HaCaT) 和人肺癌 (A549) 细胞的培养基中 6 小时和 24 小时。然后，检查线粒体活性（MTT 测定）和细胞氧化应激的诱导。暴露于金和银会降低线粒体活性。暴露于 Pt 纳米颗粒会导致细胞内活性氧 (ROS) 水平增加。相比之下，Au-PVP、Pt-PVP 和亲水性炭黑没有表现出任何效果。本研究中观察到的 Pt 纳米粒子诱导的 ROS 水平增加与我们之前的发现相矛盾，我们之前的发现是，Pt 不产生化学反应性分子。一些纳米颗粒分散体包含化学物质作为分散剂，用于工业应用。在某些情况下，分散剂可能会引起一些细胞效应。纳米粒子表面上的试剂的吸附可能是这里的一个重要因素。因此，应该仔细评估工业纳米颗粒的细胞效应。
The effects of five types of metal nanoparticles, gold (Au), silver (Ag), platinum (Pt), Au-polyvinylpyrrolidone (PVP) colloid, and Pt-PVP colloid, and two types of hydrophilic carbon black on cell behavior were examined. Stable nanoparticle dispersions were prepared and applied to the culture medium of human keratinocyte (HaCaT) and human lung carcinoma (A549) cells for 6 and 24 hr. Then, the mitochondrial activity (MTT assay) and the induction of cellular oxidative stress were examined. The exposure to Au and Ag decreased mitochondrial activity. The exposure to Pt nanoparticles induced an increase in the intracellular reactive oxygen species (ROS) level. In contrast, Au-PVP, Pt-PVP, and hydrophilic carbon black did not exhibit any effects. The observed increase in the ROS level induced by the Pt nanoparticles in this study contradicted our previous findings, in which Pt did not produce chemically reactive molecules. Some nanoparticle dispersions included chemicals as the dispersant, which is used in industrial applications. In some cases, the dispersing agent may have caused some cellular effects. Adsorption of agents on the surface of the nanoparticles may be an important factor here. Hence, the cellular effects of industrial nanoparticles should be evaluated carefully.