TMPDB: a database of experimentally-characterized transmembrane topologies
TMPDB: a database of experimentally-characterized transmembrane topologies
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DOI：
10.1093/nar/gkg020
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发表时间：
2003-01-01
影响因子：
14.9
通讯作者：
Shimizu, T
中科院分区：
文献类型：
--
作者：
Ikeda, M;Arai, M;Shimizu, T
TMPDB是一个实验表征的跨膜(TM)拓扑结构的数据库。TMPDB Release 6.2共包含302个TM蛋白序列，其中276个是α-螺旋序列，17个是β-链序列，9个α-螺旋序列有短的成孔螺旋埋入膜中。通过X射线结晶学、核磁共振、基因融合技术、取代半胱氨酸可及性方法、N-连接糖基化实验等生化方法对TMPDB的TM拓扑结构进行了实验测定。TMPDB将作为测试和/或训练数据集，用于改进已提出的TM拓扑预测方法或开发性能更高的新方法，并作为生物信息学家和生物学家更好地理解TM蛋白质的指南。TMPDB及其子集可在以下网站免费获得：http：//Bioinfo.si。广崎美国公司。Jp/类似于TMPDB/。
TMPDB is a database of experimentally-characterized transmembrane ( TM) topologies. TMPDB release 6.2 contains a total of 302 TM protein sequences, in which 276 are alpha-helical sequences, 17 beta-stranded, and 9 alpha-helical sequences with short pore-forming helices buried in the membrane. The TM topologies in TMPDB were determined experimentally by means of X-ray crystallography, NMR, gene fusion technique, substituted cysteine accessibility method, N-linked glycosylation experiment and other biochemical methods. TMPDB would be useful as a test and/or training dataset in improving the proposed TM topology prediction methods or developing novel methods with higher performance, and as a guide for both the bioinformaticians and biologists to better understand TM proteins. TMPDB and its subsets are freely available at the following web site: http: / / bioinfo.si. hirosaki- u. ac. jp/similar toTMPDB/.