Plasma MMP-9 (92 kDa-MMP) activity is useful in the follow-up and in the assessment of prognosis in breast cancer patients
Plasma MMP-9 (92 kDa-MMP) activity is useful in the follow-up and in the assessment of prognosis in breast cancer patients
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DOI：
10.1002/ijc.11288
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发表时间：
2003-09-20
影响因子：
6.4
通讯作者：
Puricelli, L
中科院分区：
文献类型：
--
作者：
Ranuncolo, SM;Armanasco, E;Puricelli, L
先前我们确定，与良性乳腺病变和健康对照相比，乳腺癌患者的血浆MMP-9活性显著升高。现在，我们分析了其在随访和这些患者的预后中的潜在有用性。MMP-9活性测定明胶定量酶谱法在优球蛋白血浆部分的46例乳腺癌患者在38个月的随访研究。在手术前（S1）、手术后1个月（S2）和每3个月采集血样。采用Kaplan-Meier曲线和考克斯分析对113例患者进行沿着56个月的无复发生存期（RFS）和总生存期（OS）分析。在分析的63%的S2中，MMP-9在手术后降低。在辅助治疗期间评估的44例患者中，与S1相比，MMP-9降低，而2例患者表现出与缺乏应答相关的增强。进一步的分析表明，在所有从未显示复发证据的患者中，血浆MMP-9活性保持较低，但在复发患者的临床检测进展前1至8个月，血浆MMP-9活性增加。Kaplan-Meier曲线表明，在乳腺癌诊断时，高水平的血浆MMP-9活性与较差的OS率相关。考克斯分析显示其与肿瘤分期和患者年龄无关。我们的研究结果表明，血浆MMP-9活性和每个患者的临床状态之间有很好的相关性，这表明其作为乳腺癌患者的随访和预后的标志物的有用性。(C)2003 Wiley-Liss，Inc.
Previously we determined that plasma MMP-9 activity was significantly elevated in breast cancer patients compared to benign mammary pathologies and healthy controls. Now we analyzed its potential usefulness in the follow-up and in the prognosis of these patients. MMP-9 activity was measured by gelatin quantitative zymography in the euglobulin plasma fraction of 46 breast cancer patients in a 38-month follow-up study. Blood samples were obtained before surgery (SI), I month after (S2) and every 3 months. The relapse-free survival (RFS) and overall survival (OS) analysis was performed along 56 months in 113 patients using the Kaplan-Meier curves and Cox analysis. In 63% of the S2 analyzed, MMP-9 decreased after surgery. In 44 patients evaluated during the adjuvant period who developed a complete response, MMP-9 decreased compared to their S 1, whereas 2 patients showed an enhancement in correlation with lack of response. Further analysis indicated that in all patients who never showed evidence of recurrence, plasma MMP-9 activity remained low, but it increased I to 8 months preceding the clinical detection of progression in those patients who relapsed. Kaplan-Meier curves indicated that high levels of plasma MMP-9 activity at the moment of breast cancer diagnosis were associated with a worse OS rate. Cox analysis showed it was not associated with tumor stage or patient's age. Our results, which show a good correlation between plasma MMP-9 activity and the clinical status of each patient, suggest its usefulness as a marker both in the follow-up and in the prognosis of breast cancer patients. (C) 2003 Wiley-Liss, Inc.