The Effects of Extracellular Calmodulin on Cell Wall Regeneration of Protoplasts and Cell Division
The Effects of Extracellular Calmodulin on Cell Wall Regeneration of Protoplasts and Cell Division
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DOI：
10.1093/oxfordjournals.pcp.a078729
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发表时间：
1995
影响因子：
4.9
通讯作者：
Daye Sun;Y. Bian;Baoshan Zhao;Liangxia Zhao;Xian-Ming Yu;Shengjun Duan
中科院分区：
文献类型：
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作者：
Daye Sun;Y. Bian;Baoshan Zhao;Liangxia Zhao;Xian-Ming Yu;Shengjun Duan
研究了抗钙调蛋白(CaM)血清、CaM拮抗剂W7-琼脂糖和外源纯CaM对白芷等植物原生质体细胞壁再生和细胞分裂的影响。抗CaM血清对原生质体细胞壁再生和第一次细胞分裂具有剂量依赖性抑制作用，而等量的免疫前血清的抑制作用远小于抗CaM血清。 CaM 拮抗剂 W7-琼脂糖也抑制第一次细胞分裂。外源纯CaM的添加增强了多种植物的原生质体细胞壁再生和细胞分裂，而等量牛血清白蛋白则无明显效果。采用酶联免疫吸附法检测正常培养基中的CaM。其含量随着培养时间的延长而增加。结果提示细胞外CaM在促进原生质体细胞壁再生和细胞分裂中发挥重要作用。讨论了细胞外 CaM 实现其作用的可能机制。
The effects of anti-calmodulin (CaM) serum, CaM antagonist W7-agarose and exogenous pure CaM on cell wall regeneration of protoplasts and cell division forAngelica dahuricaand other plants were studied. Anti-CaM serum inhibited cell wall regeneration of protoplasts and the first cell division in dose-dependent manner, while the same amount of preimmune serum had a much less inhibitory effect than anti-CaM serum. The first cell division was also inhibited by CaM antagonist W7-agarose. The addition of exogenous pure CaM enhanced cell wall regeneration of protoplasts and the cell division for several species of plants, while the same amount of bovine serum albumin had no obvious effect. CaM was detected in the normal culture medium by means of enzyme-linked immunosorbent assay. Its content increased with the culture time. The results suggest that extracellular CaM plays an important role in promoting cell wall regeneration of protoplasts and cell division. The possible mechanisms by which extracellular CaM achieves its effects are discussed.