Induced Current-Pharmacological Split Stimulation System for Neuronal Networks
Induced Current-Pharmacological Split Stimulation System for Neuronal Networks
复制标题
神经元网络的诱导电流药理分裂刺激系统
DOI：
10.1109/tbme.2013.2281079
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Jimbo Y.
中科院分区：
文献类型：
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作者：
Saito A.;Takayama Y.;Kotani K.;Jimbo Y.
磁刺激通过磁感应电流非侵入性地调节神经元活动。然而，尽管这种方法的有用性和普及性，在非刺激区域的神经元活动的刺激反应的影响是未知的。在这里，我们报告说，诱导电流诱发的反应的影响，在非刺激区域的神经元活动。我们的实验使用了一种基于Mu金属的局部感应电流刺激（LICS）系统，结合了微加工细胞培养室系统和微电极阵列（MEA）。细胞培养室系统具有连接一个中心室和八个外室的辐射微隧道，其使用软光刻和复制建模技术与SU-8光致抗蚀剂和聚二甲基硅氧烷（PDMS）制造。将大鼠皮层神经元单独培养在小室中，并通过微通道形成功能性突触连接。通过施加双相交变脉冲磁场到位于中央室的Mu金属，感应电流主要产生在培养的神经元附近，并修改神经元的活动，这是通过MEA记录。此外，我们证实，诱发反应的修改由局部药物刺激（LPS）在外室。这些结果表明，我们的系统将是有前途的工具，用于分析磁刺激相互作用的神经元活动的影响。
Magnetic stimulation noninvasively modulates neuronal activity through a magnetically induced current. However, despite the usefulness and popularity of this method, the effects of neuronal activity in the nonstimulated regions on the stimulus responses are unknown. Here, we report that the induced current-evoked responses were affected by neuronal activities in the nonstimulated regions. Our experiment used a Mu-metal-based localized induced current stimulation (LICS) system combined with the microfabricated cell culture chamber system and a microelectrode array (MEA). The cell culture chamber system has radiating microtunnels connecting one central and eight outer chambers, which were fabricated using soft lithography and a replica modeling technique with SU-8 photoresist and polydimethylsiloxane (PDMS). Rat cortical neurons were separately cultured in the chambers and formed functional synaptic connections through the microtunnels. By applying a biphasic alternating pulsed magnetic field to the Mu-metal located in the central chamber, induced currents were mainly generated near the cultured neurons and modified the neuronal activities, which were recorded through MEA. Furthermore, we confirmed that the evoked responses were modified by localized pharmacological stimulation (LPS) in the outer chambers. These results suggest that our system would be promising tool for analyzing the effect of magnetic stimulation on interacting neuronal activity.