Comprehensive Analysis of ABA Effects on Ethylene Biosynthesis and Signaling during Tomato Fruit Ripening.
Comprehensive Analysis of ABA Effects on Ethylene Biosynthesis and Signaling during Tomato Fruit Ripening.
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DOI：
10.1371/journal.pone.0154072
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Ying T
中科院分区：
文献类型：
--
作者：
Mou W;Li D;Bu J;Jiang Y;Khan ZU;Luo Z;Mao L;Ying T
阿坝在果实成熟过程中对乙烯的生物合成和信号转导具有重要的调控作用，但这两种激素之间相互作用的分子机制尚不清楚。在本研究中，外源阿坝处理明显促进果实成熟以及乙烯释放，而NDGA（去甲二氢愈创木酸，阿坝生物合成的抑制剂）的应用表现出相反的生物效应。结合RNA-seq和时程RT-PCR分析，我们的研究不仅有助于阐明阿坝在转录水平上如何调节自身，而且揭示了阿坝可能通过调节LeACS 4、LeACO 1、GR和LeETR 6等关键基因来促进乙烯的产生和反应。此外，1-MCP处理的果实阿坝暴露后立即调查表明，乙烯可能是必不可少的诱导阿坝的生物合成和信号在果实成熟的开始。此外，一些乙烯合成和敏感性的特异性转录因子（如MADS-RIN、TAGL 1、CNR和NOR）也被观察到对阿坝有反应，这表明阿坝可能通过调节这些转录因子的表达来影响乙烯的作用。我们的综合生理和分子水平的分析揭示了阿坝和乙烯之间的串扰在番茄果实成熟过程中的机制。
ABA has been widely acknowledged to regulate ethylene biosynthesis and signaling during fruit ripening, but the molecular mechanism underlying the interaction between these two hormones are largely unexplored. In the present study, exogenous ABA treatment obviously promoted fruit ripening as well as ethylene emission, whereas NDGA (Nordihydroguaiaretic acid, an inhibitor of ABA biosynthesis) application showed the opposite biological effects. Combined RNA-seq with time-course RT-PCR analysis, our study not only helped to illustrate how ABA regulated itself at the transcription level, but also revealed that ABA can facilitate ethylene production and response probably by regulating some crucial genes such as LeACS4, LeACO1, GR and LeETR6. In addition, investigation on the fruits treated with 1-MCP immediately after ABA exposure revealed that ethylene might be essential for the induction of ABA biosynthesis and signaling at the onset of fruit ripening. Furthermore, some specific transcription factors (TFs) known as regulators of ethylene synthesis and sensibility (e.g. MADS-RIN, TAGL1, CNR and NOR) were also observed to be ABA responsive, which implied that ABA influenced ethylene action possibly through the regulation of these TFs expression. Our comprehensive physiological and molecular-level analysis shed light on the mechanism of cross-talk between ABA and ethylene during the process of tomato fruit ripening.