Use of an inducible reporter gene system for the analysis of auxin distribution in the moss Physcomitrella patens
Use of an inducible reporter gene system for the analysis of auxin distribution in the moss Physcomitrella patens
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DOI：
10.1007/s00299-003-0646-1
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发表时间：
2003-08-01
影响因子：
6.2
通讯作者：
Decker, EL
中科院分区：
文献类型：
--
作者：
Bierfreund, NM;Reski, R;Decker, EL
植物激素生长素在各种生长和发育反应中起着重要作用，甚至在更古老的植物中也是如此，例如苔藓的细胞分化。然而，生长素在苔藓发育过程中的分布几乎一无所知。为了解决这个问题，我们的特点生长素分布在藓小立碗藓使用生长素诱导的报告基因系统。产生了分别由生长素诱导型启动子GH 3和DR 5驱动的表达β-葡萄糖醛酸酶（GUS）基因的稳定转基因立碗藓属植物。两种融合体在生长素诱导的启动子强度和表达动力学方面表现出显着差异。GUS在不同发育阶段的表达模式的详细表征显示，生长素浓度最高的分裂和个体发育的年轻细胞。
The plant hormone auxin plays a major role in a variety of growth and developmental responses, even in the more ancient plants-for example, cell differentiation in mosses. Nevertheless, almost nothing is known about the distribution of auxin during moss development. To address this question, we characterised auxin distribution in the moss Physcomitrella patens using auxin-inducible reporter gene systems. Stable transgenic Physcomitrella plants were produced expressing the beta-glucuronidase (GUS) gene driven by the auxin-inducible promoters GH3 and DR5, respectively. Both fusions showed remarkable differences with respect to auxin-induced promoter strength and expression kinetics. A detailed characterisation of the GUS expression pattern in different developmental stages revealed that the highest auxin concentrations were in dividing and ontogenetic young cells.