CD8+ tumor-infiltrating lymphocytes together with CD4+ tumor-infiltrating lymphocytes and dendritic cells improve the prognosis of patients with pancreatic adenocarcinoma
CD8+ tumor-infiltrating lymphocytes together with CD4+ tumor-infiltrating lymphocytes and dendritic cells improve the prognosis of patients with pancreatic adenocarcinoma
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DOI：
10.1097/00006676-200401000-00023
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发表时间：
2004-01-01
期刊：
影响因子：
2.9
通讯作者：
Katoh, H
中科院分区：
文献类型：
--
作者：
Fukunaga, A;Miyamoto, M;Katoh, H
目的：最近的研究表明，肿瘤免疫对癌症患者预后的重要性。在某些类型的癌症中，免疫组织化学分析表明，CD8(+)肿瘤浸润性淋巴细胞(TIL)的存在是决定预后的关键因素。在一个实验模型中，CD4(+)淋巴细胞和CD8(+)淋巴细胞对肿瘤免疫有显著贡献。免疫组织化学染色检测CD4、CD8和S100蛋白的表达，并进行光镜分析。CD4(+)组和CD8(+)组患者的比例分别为59%(47/80)和25%(16/80)。当分为CD_4/8(+/+)、CD_4/8(+/-)、CD_4/8(-/+)和CD_4/8(-/-)四组时，CD_4/8(+/+)组总生存率(13例)显著高于其他组(67例；P=0.0098)。CD4/8(+/+)状态与肿瘤深度、TNM分期呈负相关。多因素分析显示，CD4/8(+/+)状态是影响预后的独立因素。CD_4/8(+/+)组肿瘤浸润性S100蛋白阳性细胞数也显著高于其他组(P=0.0084)。结论：在胰腺癌患者中，CD_4(+)T淋巴细胞和CD_8(+)T淋巴细胞的存在可作为判断胰腺癌术后预后的良好指标。
Objective: Recent studies have demonstrated the importance of tumor immunity for a cancer patient's prognosis. In some types of cancer, it has been shown through immunohistochemical analysis that the existence of CD8(+) tumor-infiltrating lymphocytes (TILs) is a crucial factor in determining prognosis. In an experimental model, CD4(+) lymphocytes together with CD8(+) lymphocytes contributed significantly to tumor immunity.Methods: Specimens were taken from 80 surgically resected pancreatic adenocarcinomas between 1992 and 1999. Immunohistochemical staining of CD4, CD8, and S100 protein was performed, and the levels of these proteins were determined by microscopic analysis. The percentages of patients in the CD4(+) and CD8(+) groups were 59% (47/80) and 25% (16/80), respectively. When separated into 4 groups, CD4/8(+/+), CD4/8(+/-), CD4/8(-/+) and CD4/8(-/-), the overall survival rate was significantly higher in CD4/8(+/+) patients ( 13 cases) compared with those in all other groups combined ( 67 cases; P=0.0098). CD4/8(+/+) status was negatively correlated with tumor depth and TNM stage. Multivariate analyses showed that CD4/8(+/+) status was an independent favorable prognostic factor. The number of tumor-infiltrating S100 protein positive cells was also significantly higher in the CD4/8(+/+) group than in others (P=0.0084).Conclusions: In pancreatic adenocarcinoma, the presence of CD4(+) TILs together with CD8(+) TILs serves as a good indicator of the patient's outcome after surgical treatment.