Luminal surfaces of fetal rat alveolar type II and Clara lung cells react with antibody to Heymann nephritis antigen.
Luminal surfaces of fetal rat alveolar type II and Clara lung cells react with antibody to Heymann nephritis antigen.
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胎鼠 II 型肺泡和 Clara 肺细胞的管腔表面与 Heymann 肾炎抗原抗体发生反应。
DOI：
10.1002/ppul.1950100407
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发表时间：
1991
影响因子：
3.1
通讯作者：
Makker,SP
中科院分区：
文献类型：
--
作者：
Kotas,RV;Kanalas,JJ;Tio,FO;Makker,SP
本文应用与肾抗原gp 600反应的亲和纯化抗体，检测了胎鼠肺组织中肺细胞的免疫反应性。在免疫电子显微镜，我们发现，该反应是专门定位于管腔表面的克拉拉细胞，如前所述，肺泡II型细胞（ATII）膜。在I型细胞、内皮细胞或任何其他肺细胞或血细胞上或中未观察到免疫反应。这些研究表明，抗gp 600免疫选择可用作识别和定量大鼠肺组织中Clara和ATII细胞的标志物。小儿肺科1991; 10：260-266.
We have used affinity purified antibody, which reacts with the renal antigen gp600, to examine the immunoreactivity of pulmonary cells in the quick frozen lung tissue of fetal rats. On immunoelectron microscopy, we found that the reaction was specifically localized to the luminal surfaces of Clara cell and, as previously reported, to alveolar type II cell (ATII) membranes. No immunoreaction was seen on or in type I cells, endothelial cells or any other lung or blood cell. These studies suggest that the anti‐gp600 immunoselection can be used as a marker to identify and quantify Clara and ATII cells in rat lung tissue. Pediatr Pulmonol 1991; 10:260–266.