Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
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DOI：
10.1093/femsec/fiw125
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发表时间：
2016-08-01
影响因子：
4.2
通讯作者：
Coppotelli, Bibiana M.
中科院分区：
文献类型：
--
作者：
Festa, Sabrina;Macchi, Marianela;Coppotelli, Bibiana M.
研究了狮身人掌的生物强化效果。研究了AM菌株在不同土壤微生物群落、原始土壤(PS)、长期污染土壤(IPK)和新近污染土壤(Phe)上的生物修复效果，重点研究了细菌群落对接种的响应生态。AM菌株基因组草稿编码了芳香烃和脂肪族碳氢化合物代谢的基因。在PHE中，接种提高了整个处理过程中菲的消除，而在IPK中，没有观察到任何多环芳烃的降解。通过焦磷酸测序分析，我们观察到接种成功地增加了两种污染微生物的丰富度和多样性，因此，除了多环芳烃降解的改善之外，我们观察到了接种剂建立的线索，这表明它可能使用其他资源来生存。另一方面，接种不影响PS的细菌群落。在两种污染的微生物群中，培养条件使放线菌目和鞘氨醇单胞菌目急剧增加，而接种导致放线菌目的相对减少。接种多种多样的PS和Phe微生物群，虽然这些属之间可能存在协同作用，但同时增加了鞘氨醇单胞目、伯克霍尔德雷亚目和根瘤目；我们的结果表明，这与多环芳烃的降解没有直接关系。一种生物强化技术对不同土壤微生物群的影响表明，接种能够增加土壤微生物群落的丰富度和多样性，对土壤生态产生有利的影响，而不利于生物修复过程。
The effect of bioaugmentation with Sphingobium sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on Actinomycetales and Sphingomonadales orders, while inoculation caused a relative decline of Actinomycetales. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of Sphingomonadales, Burkholderiales and Rhizobiales orders, although it may exist a synergy between those genera; our results suggest that this would not be directly related to PAH degradation.The effect of a bioaugmentation technique on different soils microbiomes showed that inoculation managed to increase the richness and diversity producing a beneficial effect on soil ecology regardless of favouring the process of bioremediation.The effect of a bioaugmentation technique on different soils microbiomes showed that inoculation managed to increase the richness and diversity producing a beneficial effect on soil ecology regardless of favouring the process of bioremediation.