A cell-free protein-producing gel
A cell-free protein-producing gel
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DOI：
10.1038/nmat2419
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发表时间：
2009-05-01
期刊：
影响因子：
41.2
通讯作者：
Luo, Dan
中科院分区：
文献类型：
--
作者：
Park, Nokyoung;Um, Soong Ho;Luo, Dan
蛋白质是重要的生物材料，通常在活细胞中产生。在这里，我们展示了一种新型的DNA水凝胶，它能够在没有任何活细胞的情况下产生功能蛋白质。这种产生蛋白质的凝胶（称为“P-凝胶系统”或“P-凝胶”）由作为凝胶支架的一部分的基因组成。这是首次将水凝胶用于生产蛋白质。其效率比目前基于溶液的系统高出约300倍。就体积产率而言，P-凝胶产生高达5 mg ml（-1）的功能蛋白。高效率和高产量背后的机制包括改善的基因稳定性，更高的局部浓度和更快的酶周转率，这是由于基因之间的距离更近。我们已经测试了总共16种不同的P-凝胶，并成功地生产了所有16种蛋白质，包括膜蛋白和毒性蛋白，这表明P-凝胶系统可以作为一种通用的蛋白质生产技术。
Proteins are important biomaterials and are generally produced in living cells. Here, we show a novel DNA hydrogel that is capable of producing functional proteins without any living cells. This protein-producing gel (termed 'the P-gel system' or 'P-gel') consists of genes as part of the gel scaffolding. This is the first time that a hydrogel has been used to produce proteins. The efficiency was about 300 times higher than current, solution-based systems. In terms of volumetric yield, the P-gel produced up to 5 mg ml(-1) of functional proteins. The mechanisms behind the high efficiency and yield include improved gene stability, higher local concentration and a faster enzyme turnover rate due to a closer proximity of genes. We have tested a total of 16 different P-gels and have successfully produced all 16 proteins including membrane and toxic proteins, demonstrating that the P-gel system can serve as a general protein production technology.