Release of RNA polymerase from vero cell mitochondria after herpes simplex virus type 1 infection.
Release of RNA polymerase from vero cell mitochondria after herpes simplex virus type 1 infection.
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1 型单纯疱疹病毒感染后，vero 细胞线粒体中 RNA 聚合酶的释放。
DOI：
10.1128/jvi.64.1.450-452.1990
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发表时间：
1990
影响因子：
5.4
通讯作者：
Lehman,IR
中科院分区：
文献类型：
--
作者：
Tsurumi,T;Lehman,IR
用单纯疱疹病毒1型感染Vero细胞导致可溶性提取物中出现DNA引发酶活性。部分纯化的酶，Mr约为100，000，在对α-鹅膏蕈碱的抗性、pH曲线、Mg 2+依赖性、盐敏感性和对Vero细胞线粒体RNA聚合酶催化核心的KmATP方面是相同的。此外，合成的产物是预期的RNA聚合酶而不是DNA引发酶的产物。由于该酶不存在于未感染的Vero细胞的可溶性提取物中，我们推测疱疹病毒感染的细胞提取物中RNA聚合酶的特异性外观是由感染诱导的线粒体膜破坏引起的，然后将酶释放到胞质溶胶中。
Infection of Vero cells with herpes simplex virus type 1 results in the appearance in soluble extracts of a DNA primase activity. The partially purified enzyme, Mr, approximately 100,000, is identical in resistance to alpha-amanitin, pH profile, Mg2+ dependence, salt sensitivity, and KmATP to the catalytic core of Vero cell mitochondrial RNA polymerase. Moreover, the products synthesized are those expected of an RNA polymerase rather than a DNA primase. Inasmuch as the enzyme is not present in soluble extracts of uninfected Vero cells, we presume that the specific appearance of RNA polymerase in extracts of herpesvirus-infected cells results from infection-induced disruption of the mitochondrial membrane, followed by release of the enzyme into the cytosol.