Evidence for bacteriophage activity causing community and performance changes in a phosphorus-removal activated sludge
Evidence for bacteriophage activity causing community and performance changes in a phosphorus-removal activated sludge
复制标题
DOI：
10.1111/j.1574-6941.2010.00967.x
复制
发表时间：
2010-12-01
影响因子：
4.2
通讯作者：
Bond, Philip L.
中科院分区：
文献类型：
--
作者：
Barr, Jeremy J.;Slater, Frances R.;Bond, Philip L.
众所周知，细菌在生物地球化学循环和生物技术过程中发挥着重要作用，但对噬菌体对这些过程的影响知之甚少。研究宿主-噬菌体相互作用的一个主要障碍是细菌和它们的噬菌体通常不能在纯培养物中获得。在这项研究中，我们发现颗粒状实验室规模的废水处理反应器的除磷性能出乎意料地下降。通过FISH，透射电子显微镜和蛋白质组学的研究，我们假设未培养的候选菌“积累杆菌”的噬菌体感染是导致性能下降的原因。进一步的实验表明，从先前失败的反应器中获得的假定富含噬菌体的上清液添加到除磷反应器中，导致颗粒状和絮状活性污泥中积累杆菌的丰度降低。这与噬菌体样颗粒的增加和磷去除性能的下降相吻合。攻击后颗粒污泥没有恢复，但絮状污泥恢复了蓄积菌数量和除磷性能。这些发现表明，噬菌体可能在决定活性污泥中细菌群落的结构和功能方面发挥重要作用。
Bacteria are known to play important roles in biogeochemical cycles and biotechnology processes, but little is known about the influence of bacteriophage on these processes. A major impediment to the study of host-bacteriophage interactions is that the bacteria and their bacteriophage are often not available in a pure culture. In this study, we detected an unexpected decline in the phosphorus-removal performance of a granular laboratory-scale wastewater treatment reactor. Investigations by FISH, transmission electron microscopy and proteomics led us to hypothesize that a bacteriophage infection of the uncultured Candidatus 'Accumulibacter phosphatis' was responsible for the decline in performance. Further experiments demonstrated that the addition of a putative bacteriophage-rich supernatant, obtained from the previous failed reactor to phosphorus-removal reactors, caused a decrease in the abundance of Accumulibacter in both granular and floccular activated sludges. This coincided with increases in bacteriophage-like particles and declining phosphorus-removal performance. The granular sludge did not recover after the attack, but the floccular sludge regained Accumulibacter numbers and phosphorus-removal performance. These findings suggest that bacteriophage may play a significant role in determining the structure and function of bacterial communities in activated sludges.