A semi-integrated method for the determination of enzyme kinetic parameters and graphical representation of the Michaelis-Menten equation.
A semi-integrated method for the determination of enzyme kinetic parameters and graphical representation of the Michaelis-Menten equation.
复制标题
用于测定酶动力学参数和米氏方程图形表示的半集成方法。
DOI：
10.1016/0003-2697(84)90442-1
复制
发表时间：
1984
影响因子：
2.9
通讯作者：
Chance,B
中科院分区：
文献类型：
--
作者：
Naqui,A;Chance,B
本文提出了一种测定酶动力学参数(Kmand V)和米氏方程图形表示的半集成方法，即测定初始反应速率(V)随初始底物浓度([S]0)的变化。该方法基于作为初始底物浓度的函数来确定耗尽初始底物浓度的一半所需的时间。讨论了该方法的优点和局限性。
A semi-integrated method for the determination of the enzyme kinetics parameters (Kmand V) and graphical representation of the Michaelis-Menten equation is proposed as a variation of determination of initial reaction rate (v) as a function of initial substrate concentration ([S]0). The method is based on the determination of the time required to exhaust half of the initial substrate concentration as a function of the initial substrate concentration. The advantages and limitations of this method are discussed.