13C pulse-labeling assessment of the community structure of active fungi in the rhizosphere of a genetically starch-modified potato (Solanum tuberosum) cultivar and its parental isoline
13C pulse-labeling assessment of the community structure of active fungi in the rhizosphere of a genetically starch-modified potato (Solanum tuberosum) cultivar and its parental isoline
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DOI：
10.1111/j.1469-8137.2012.04089.x
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发表时间：
2012-05-01
期刊：
影响因子：
9.4
通讯作者：
van Veen, J. A.
中科院分区：
文献类型：
--
作者：
Hannula, S. E.;Boschker, H. T. S.;van Veen, J. A.
本研究旨在利用稳定同位素探测（SIP）技术，了解一种聚支链淀粉转基因马铃薯（Solanum tuberosum）及其亲本等系根系向土壤真菌群落的碳流量。通过稳定同位素探针（PLFA-SIP）的RNA/磷脂脂肪酸分析，在标记开始后的3个时间点（1、5和12 d）对从植物中获得13C的微生物进行了评估。采用RT-qPCR和末端限制性片段长度多态性（T-RFLP）对子囊菌门、担子菌门和肾小球菌门进行群落分析。子囊菌和肾小球菌最早在标记后的第1天和第5天就从植物中获得了碳，而担子菌积累标记碳的速度较慢。转基因品种的碳分配速率与其亲本品种不同，从而影响了土壤真菌群落。我们得出结论，腐养真菌和菌根真菌都能快速代谢从根部流入根际的有机基质，在所研究的真菌门中，在较低的系统发育水平上对根源化合物的利用存在很大差异，并且转基因植物与其亲本栽培品种的根际活跃群落存在差异，这是由于植物碳流的差异造成的。
The aim of this study was to gain understanding of the carbon flow from the roots of a genetically modified (GM) amylopectin-accumulating potato (Solanum tuberosum) cultivar and its parental isoline to the soil fungal community using stable isotope probing (SIP). The microbes receiving 13C from the plant were assessed through RNA/phospholipid fatty acid analysis with stable isotope probing (PLFA-SIP) at three time-points (1, 5 and 12 d after the start of labeling). The communities of Ascomycota, Basidiomycota and Glomeromycota were analysed separately with RT-qPCR and terminal restriction fragment length polymorphism (T-RFLP). Ascomycetes and glomeromycetes received carbon from the plant as early as 1 and 5 d after labeling, while basidiomycetes were slower in accumulating the labeled carbon. The rate of carbon allocation in the GM variety differed from that in its parental variety, thereby affecting soil fungal communities. We conclude that both saprotrophic and mycorrhizal fungi rapidly metabolize organic substrates flowing from the root into the rhizosphere, that there are large differences in utilization of root-derived compounds at a lower phylogenetic level within investigated fungal phyla, and that active communities in the rhizosphere differ between the GM plant and its parental cultivar through effects of differential carbon flow from the plant.