Intracellular Antioxidant Detoxifying Effects of Diosmetin on 2,2-Azobis(2-amidinopropane) Dihydrochloride (AAPH)-Induced Oxidative Stress through Inhibition of Reactive Oxygen Species Generation
Intracellular Antioxidant Detoxifying Effects of Diosmetin on 2,2-Azobis(2-amidinopropane) Dihydrochloride (AAPH)-Induced Oxidative Stress through Inhibition of Reactive Oxygen Species Generation
复制标题
DOI：
10.1021/jf502359x
复制
发表时间：
2014-08-27
影响因子：
6.1
通讯作者：
Ren, Jiaoyan
中科院分区：
文献类型：
--
作者：
Liao, Wenzhen;Ning, Zhengxiang;Ren, Jiaoyan
采用细胞抗氧化活性(CAA)法、2，2-偶氮二(2-氨基丙烷)二盐酸盐(AAPH)诱导的红细胞溶血实验和CuCl2诱导的血浆氧化实验评价Diosmetin的细胞内抗氧化活性。结果表明，Diosmetin具有较强的细胞抗氧化活性(EC50=7.98mU·m-1，CAA值=58 mU·m-1·Qe/100 m u·m-1)。研究还发现，Diosmetin可有效抑制AAPH诱导的红细胞溶血(100µg/mL时抑制率为91.0%)和CuCl2诱导的血浆氧化反应，其机制是抑制细胞内ROS的产生。Diosmetin能显著恢复AAPH诱导的细胞内抗氧化酶(SOD、GPx和CAT)活性的升高，并抑制细胞内丙二醛(MDA)的生成。因此，Diosmetin的细胞内抗氧化解毒机制与非酶防御系统和酶防御系统有关。
The intracellular antioxidant activities of diosmetin were evaluated by cellular antioxidant activity (CAA) assay, 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced erythrocyte hemolysis assay and cupric chloride (CuCl2)-induced plasma oxidation assay. The results showed that diosmetin exhibits strong cellular antioxidant activity (EC50 = 7.98 mu mol, CAA value = 58 mu mol QE/100 mu mol). It was also found that diosmetin treatment could effectively attenuate AAPH-induced erythrocyte hemolysis (91.0% inhibition at 100 mu g/mL) and CuCl2-induced plasma oxidation through inhibition of intracellular reactive oxygen species (ROS) generation. Diosmetin could significantly restore AAPH-induced increase of intracelluar antioxidant enzyme (SOD, GPx, and CAT) activities to normal levels, as well as inhibit intracellular malondialdehyde (MDA) formation. Thus, the intracellular antioxidant detoxifying mechanism of diosmetin is associated with both nonenzymatic and enzymatic defense systems.