FLUORESCENCE OF CHLOROPHYLL IN PHOTOSYNTHETIC SYSTEMS .3. EMISSION AND ACTION SPECTRA OF FLUORESCENCE - 3 EMISSION BANDS OF CHLOROPHYLL A AND ENERGY TRANSFER BETWEEN 2 PIGMENT SYSTEMS
FLUORESCENCE OF CHLOROPHYLL IN PHOTOSYNTHETIC SYSTEMS .3. EMISSION AND ACTION SPECTRA OF FLUORESCENCE - 3 EMISSION BANDS OF CHLOROPHYLL A AND ENERGY TRANSFER BETWEEN 2 PIGMENT SYSTEMS
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DOI：
10.1016/0926-6585(66)90059-8
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发表时间：
1966-01-01
期刊：
影响因子：
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通讯作者：
TAKAMIYA, A
中科院分区：
文献类型：
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作者：
MURATA, N;NISHIMUR.M;TAKAMIYA, A
研究了菠菜叶绿体、绿藻、条斑紫菜、紫球藻和其他光合作用生物的光合色素，特别是叶绿素a的荧光发射光谱和作用光谱，以阐明这些色素在荧光和能量传递中的行为。在长划线196度处，大部分材料的叶绿素a在684m、695m和710-735m处(分别命名为F684、F695和F-L)有3个发射带。使命和作用光谱表明，F684和F695被藻胆蛋白或叶绿素b(色素系统II)吸收的光激发，而被叶绿素a(色素系统I)吸收的光激发效率较低或根本不被激发。F-1在这两个波长区都被激发。色素体系I对F-1的吸光效率高于F684和F695，色素体系n对F684和F695的吸光效率高于F-1。在[长划线]196[度]下，菠菜叶绿体的荧光量子产率高达0.75。在这个温度下，F-1大约是。总量子的3/4，即使在475米[亩]光的激发下也是如此。结果表明，色素系统II中含有释放F684和F695的两种形式的叶绿素a，而色素系统I中含有F-1，能量从色素系统II传递到色素系统I，至少在[长划线]196度，而且可能在生理温度下也是如此。
The emission and action spectra of fluorescence of photosynthetic pigments, especially of chlorophyll a, were studied in spinach chloroplasts, Anacystls nidulans, Porphyra yezoensia, Porphyridium cruentum and other photosynthetic organisms at -196[degree], to elucidate the behavior of the pigments in fluorescence and energy transfer. At[long dash]196[degree], in most of the materials used, 3 emission bands of chlorophyll a were observed at 684 m[mu], 695 m[mu] and 710-735 m[mu] (designated as F684, F695 and F-l). The mission and action spectra showed that F684 and F695 were excited by the light absorbed by phycobilins or chlorophyll b (pigment system II) and were excited less effectively, or not at all, by the light absorbed by chlorophyll a (pigment system I). F-1 was excited by both wavelength regions. The light absorbed by pigment system I was more effective for F-1 than for F684 and F695, and the light absorbed by pigment system n was more effective for F 684 and F695 than for F-1. A quantum yield of fluorescence as high as 0.75 was obtained in spinach chloroplasts at[long dash]196[degree]. At this temperature, F-1 amounted to approx. 3/4 of the total quanta emitted, even upon excitation by 475-m[mu] light. It was concluded that 2 forms of chlorophyll a emitting F684 and F695 are contained in pigment system II, while F-1 is involved in pigment system I. Energy is transferred from pigment system II to I, at least at[long dash]196[degree] and presumably also at physiological temperatures.