Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging
Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging
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DOI：
10.1016/j.biomaterials.2004.01.047
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发表时间：
2004-09-01
期刊：
影响因子：
14
通讯作者：
Hutmacher, DW
中科院分区：
文献类型：
--
作者：
Jones, AC;Milthorpe, B;Hutmacher, DW
本文阐述了显微计算机断层扫描（micro-CT）研究组织工程骨生长过程的实用性。描述了一种用于三维（3D）成像和可视化生物材料的微型CT设备。该设施是能够采集3D图像，由2000（3）体素的标本高达60毫米的范围与分辨率下降到2妈妈。这使得组织工程材料的3D结构能够在三个数量级的细节上成像。通过对人股骨皮质骨（远端骨干）内的哈弗氏网络和骨长入多孔支架的不同分辨率成像，证明了微型CT的能力。相位识别结合3D可视化使人们能够观察皮质骨小管系统的复杂拓扑结构。以1cm的尺度和10 μ m的分辨率对组织工程化骨进行成像，可以可视化骨向聚合物支架中的复杂向内生长。在2 μ m分辨率下的进一步成像允许观察骨超微结构。这些观察结果说明了断层扫描优于传统技术的优点，用于表征骨形态和互连性，并对当前的组织形态测量技术起到补充作用。(C)2004爱思唯尔有限公司保留所有权利。
This paper illustrates the utility of micro-computed tomography (micro-CT) to study the process of tissue engineered bone growth. A micro-CT facility for imaging and visualising biomaterials in three dimensions (3D) is described. The facility is capable of acquiring 3D images made up of 2000(3) voxels on specimens up to 60 mm in extent with resolutions down to 2 mum. This allows the 3D structure of tissue engineered materials to be imaged across three orders of magnitude of detail. The capabilities of micro-CT are demonstrated by imaging the Haversian network within human femoral cortical bone (distal diaphysis) and bone ingrowth into a porous scaffold at varying resolutions. Phase identification combined with 3D visualisation enables one to observe the complex topology of the canalicular system of the cortical bone. Imaging of the tissue engineered bone at a scale of 1 cm and resolutions of 10 mum allows visualisation of the complex ingrowth of bone into the polymer scaffold. Further imaging at 2 mum resolution allows observation of bone ultra-structure. These observations illustrate the benefits of tomography over traditional techniques for the characterisation of bone morphology and interconnectivity and performs a complimentary role to current histomorphometric techniques. (C) 2004 Elsevier Ltd. All rights reserved.