Midbrain dopaminergic neurons in the mouse: co-localization with Calbindin-D28K and calretinin.
Midbrain dopaminergic neurons in the mouse: co-localization with Calbindin-D28K and calretinin.
复制标题
小鼠中脑多巴胺能神经元：与 Calbindin-D28K 和 calretinin 共定位。
DOI：
10.1016/0306-4522(96)00228-x
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
German,DC
中科院分区：
文献类型：
--
作者：
Liang,CL;Sinton,CM;German,DC
钙结合蛋白 Calbindin-D28k 和 calretinin 与大鼠和猴子的一些中脑多巴胺能神经元中的多巴胺共定位；本研究试图检查小鼠的共定位模式。对酪氨酸羟化酶（多巴胺能细胞标记物）和 Calbindin-D28kor calretinin 使用双重免疫荧光染色程序。在四个头尾水平检查中脑多巴胺能神经元，并测定 A8 核（红后区）、A9 核（黑质致密部、网状部和外侧部）和 A10 核（旁黑核、腹侧被盖区、束间核、中央线状核）中同时含有酪氨酸羟化酶和两种钙结合蛋白之一的细胞百分比。核）。这两种钙结合蛋白类似地分布在由 A8、A9 和 A10 核组成的几个核组中的中脑多巴胺能神经元中。钙结合蛋白存在于大多数（50-100%）A10 核神经元中，而在 A8 和 A9 核（黑质外侧部除外）中，不到 40% 的细胞含有任一钙结合蛋白。小鼠的共定位模式与报道的大鼠和猴子的共定位模式相似。钙结合蛋白标记了帕金森病 1-甲基-4-苯基-1,2,3,6-四氢吡啶小鼠模型中不易发生变性的中脑多巴胺能神经元群体。
The calcium-binding proteins Calbindin-D28kand calretinin are co-localized with dopamine in some of the midbrain dopaminergic neurons in the rat and monkey; the present study sought to examine the pattern of co-localization in the mouse. Double immunofluorescence staining procedures were used for tyrosine hydroxylase (a dopaminergic cell marker) and Calbindin-D28kor calretinin. Midbrain dopaminergic neurons were examined at four rostrocaudal levels, and the percentage of cells that contained both tyrosine hydroxylase and either of the two calcium-binding proteins was determined in nucleus A8 (retrorubral field), nucleus A9 (substantia nigra pars compacta, pars reticulata and pars lateralis), and nucleus A10 (nucleus paranigralis, ventral tegmental area, interfascicular nucleus, central linear nucleus). The two calcium-binding proteins were similarly distributed in midbrain dopaminergic neurons in the several nuclear groups that comprise nuclei A8, A9 and A10. The calcium-binding proteins were found in the majority (50–100%) of nucleus A10 neurons, whereas in nuclei A8 and A9 (except for the substantia nigra pars lateralis) less than 40% of the cells contained either calcium-binding protein. The pattern of co-localization in the mouse is similar to that reported for the rat and monkey. The calcium-binding proteins mark the population of midbrain dopaminergic neurons that are less vulnerable to degeneration in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson's disease.