INHERITED PERSISTENCE OF IMMATURE TYPE PYRUVATE-KINASE AND HEXOKINASE ISOZYMES IN DOG ERYTHROCYTES
INHERITED PERSISTENCE OF IMMATURE TYPE PYRUVATE-KINASE AND HEXOKINASE ISOZYMES IN DOG ERYTHROCYTES
复制标题
DOI：
10.1016/0305-0491(89)90328-3
复制
发表时间：
1989-01-01
影响因子：
2.2
通讯作者：
MAEDE, Y
中科院分区：
文献类型：
--
作者：
INABA, M;MAEDE, Y
1.高钾和低钾犬的红细胞丙酮酸激酶（EC 2.7.1.40）和己糖激酶（EC 2.7.1.1）以多种形式存在，可通过电泳和离子交换色谱分离。R2型丙酮酸激酶被确定为犬红细胞中的年轻型酶，被证明是高K细胞中丙酮酸激酶的主要形式。2. M2型丙酮酸激酶是红系细胞中的一种原型同工酶，存在于高K犬红细胞以及高K和低K犬网织红细胞中。3.高钾红细胞己糖激酶的同工酶分析也显示了类似的配置文件，获得低K网织红细胞。4.这些结果似乎反映了高K红细胞的不成熟，这表明在高K犬红系细胞增殖的早期阶段可能发生异常的细胞分化或成熟。
1. Red cell pyruvate kinase (EC 2.7.1.40) and hexokinase (EC 2.7.1.1) in high and low potassium (K) dogs were shown to exist as multiple forms which were separable by electrophoresis and ion-exchange chromatography. The R2-type pyruvate kinase, which was determined to be a young type enzyme in canine red cells, was shown to be the predominant form of pyruvate kinase in high K cells. 2. The M2-type pyruvate kinase, a prototype isozyme in erythroid cells, existed in high K dog erythrocytes as well as in high K and low K dog reticulocytes. 3. Isozyme analysis of high K red cell hexokinase also showed a profile similar to that obtained for low K reticulocytes. 4. These results seem to reflect the immaturity of high K erythrocytes, which suggest that an abnormal cell differentiation or maturation may occur at an early stage of erythroid cell proliferation in high K dogs.