STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE
STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE
复制标题
电子显微镜观察到的病毒的结构和发育
DOI：
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发表时间：
1954
影响因子：
15.3
通讯作者：
D. Moore
中科院分区：
文献类型：
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作者：
C. Morgan;S. A. Ellison;H. M. Rose;D. Moore
通过电子显微镜在受感染的鸡胚绒毛尿囊膜切片中观察到单纯疱疹病毒。从相对较厚的切片中去除包埋的甲基丙烯酸酯可以看到大量的病毒颗粒，但导致细胞成分的广泛改变以及病毒结构的不同变形。这种扭曲的特点是颗粒的破坏和中心体的损失，导致环形或空壳形式。在超薄切片中揭示了病毒的内部结构，其中包埋的塑料未被去除。受感染细胞的细胞核含有小而致密的初级体（直径为 30 至 40 mμ）以及由单膜（直径为 70 至 100 mμ）包围的稍大且密度较小的颗粒（直径为 40 至 50 mμ）。在细胞质中，大多数颗粒具有双层外膜（直径 120 至 130 mμ）。有人认为，病毒发育的初始位点仅限于细胞核，在细胞核中形成初级体并被单一外膜包围。一旦释放到细胞质中，这些颗粒似乎获得了第二层外膜，并且可能代表了成熟的病毒。
Herpes simplex virus was visualized by the electron microscope in sections of infected chorioallantoic membrane of chicken embryos. Removal of the embedding methacrylate from relatively thick sections permitted large numbers of viral particles to be seen but caused extensive alteration of cellular components as well as variable distortion of viral structure. This distortion was characterized by disruption of particles and loss of central bodies, resulting in ring or empty shell forms. An inner structure of the virus was revealed in ultrathin sections from which the embedding plastic was not removed. The nuclei of infected cells contained small, dense, primary bodies (30 to 40 mµ in diameter) as well as slightly larger and less dense particles (40 to 50 mµ in diameter) surrounded by a single membrane (70 to 100 mµ in diameter). In the cytoplasm most of the particles possessed a double outer membrane (120 to 130 mµ in diameter). It is suggested that the initial site of viral development is restricted to the nucleus where primary bodies form and become enclosed by a single outer membrane. Upon release into the cytoplasm these particles appear to acquire a second outer membrane and presumably represent the mature virus.