Regulation of glucocorticosteroid receptor expression in rat hippocampal cell cultures by nerve growth factor.
Regulation of glucocorticosteroid receptor expression in rat hippocampal cell cultures by nerve growth factor.
复制标题
神经生长因子对大鼠海马细胞培养物中糖皮质激素受体表达的调节。
DOI：
10.1016/s0304-3940(96)12454-x
复制
发表时间：
1996
影响因子：
2.5
通讯作者：
Meaney,MJ
中科院分区：
文献类型：
--
作者：
Sarrieau,A;O'Donnell,D;Alonso,R;Meaney,MJ
Dispersed hippocampal cells cultured in serum-free conditions were used to study the effects of nerve growth factor (NGF) on the expression of type I (mineralocorticosteroid or MR) and type II (glucocorticosteroid or GR) corticosteroid receptors. Cells, plated at a density of 1.2 x 106cells/ml in 60 mm Petri dishes, were mainly identified as neurons (90–95%) and maintained for at least 2 weeks. A 7-day treatment with 10–50 ng NGF/ml induced a concentration-dependent decrease of GR binding (40% decrease) compared to entreated cells. In contrast, MR density was unaffected by a 7-day treatment with 50 ng NGF/ml. Data are discussed as possible direct and/or indirect effects of NGF at the level of both neuronal and glial cells.
