Evaluation of in vitro hemolysis and platelet activation of a newly developed maglev LVAD and two clinically used LVADs with human blood
Evaluation of in vitro hemolysis and platelet activation of a newly developed maglev LVAD and two clinically used LVADs with human blood
复制标题
DOI：
10.1111/aor.13471
复制
发表时间：
2019-09-01
影响因子：
2.4
通讯作者：
Wu, Zhongjun J.
中科院分区：
文献类型：
--
作者：
Berk, Zachary B. K.;Zhang, Jiafeng;Wu, Zhongjun J.
体外溶血试验是评价左心室辅助装置（LVAD）血液相容性的重要指标之一。临床相关的操作条件和适当的血液检测对于推断潜在临床用途的体外数据至关重要。这项体外研究的目的是使用新鲜人血评估和比较新开发的磁悬浮（磁悬浮）LVAD（CH-VAD）与两种临床使用的LVAD（HVAD和HeartMate II（HMII））的溶血性能。构建了一个小体积（约300 mL）体外循环流动回路，每个LVAD在标称或报告的临床运行速度下产生的流量为4.5 L/min。血液在回路中循环4小时，并在基线和每小时采集样本。采用分光光度法测定每小时血样中的血浆游离血红蛋白（PFH）浓度。计算标准化溶血指数（NIH）以比较CH-VAD和两种参考LVAD的溶血性能。用流式细胞术测量血小板活化。每个器械的实验测试重复至少7次。本研究的数据显示，所有三种LVAD均产生非常低的溶血（NIH
In vitro hemolysis testing remains one of the most important performance measures to judge the hemocompatibility of a left ventricular assist device (LVAD). Clinically relevant operating conditions and appropriate testing blood are essential to infer in vitro data for potential clinical use. This in vitro study was carried out to evaluate and compare the hemolytic performance of a newly developed magnetically levitated (maglev) LVAD (CH-VAD) with two clinically used LVADs (HVAD and HeartMate II (HMII)) using fresh human blood. A small volume (~300 mL) in vitro circulating flow loop was constructed with a LVAD generated flow of 4.5 L/min at the nominal or reported clinical operating speed for each LVAD. The blood was circulated in the loop for 4 hours with samples drawn at baseline and hourly. Plasma-free hemoglobin (PFH) concentrations in the hourly blood samples were determined with spectrophotometry. Normalized index of hemolysis (NIH) was calculated to compare the hemolytic performance of the CH-VAD and the two reference LVADs. Platelet activation was measured with flow cytometry. The experimental test for each device was repeated at least 7 times. The data from this study showed that all the three LVADs generated very low hemolysis (NIH