Paucity of CD34-positive cells and increased expression of high-mobility group box 1 in coronary thrombus with type 2 diabetes mellitus
Paucity of CD34-positive cells and increased expression of high-mobility group box 1 in coronary thrombus with type 2 diabetes mellitus
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DOI：
10.1016/j.atherosclerosis.2012.07.027
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发表时间：
2012-10-01
期刊：
影响因子：
5.3
通讯作者：
Asada, Yujiro
中科院分区：
文献类型：
--
作者：
Yamashita, Atsushi;Nishihira, Kensaku;Asada, Yujiro
为了检查急性冠状动脉血栓中cd34阳性细胞和核内因子的存在，我们比较了2型糖尿病（DM, n = 21）和非DM （n = 29）患者的血栓。免疫组化染色显示所有血栓中存在血小板、纤维蛋白、红细胞、中性粒细胞、细胞外高迁移率组盒1 （HMGB-1）和组蛋白H3。在糖尿病患者的血栓中，cd34呈椭圆形或扁平状的细胞明显增多，hmgb -1呈扁平状的细胞表达外核苷三磷酸二磷酸水解酶（一种血小板聚集抑制剂）。cd34阳性细胞数与血清葡萄糖、糖化血红蛋白呈负相关，hmgb -1阳性面积与血清葡萄糖呈正相关。2型糖尿病患者急性冠状动脉血栓中cd34阳性细胞的缺乏和HMGB-1的高水平表达可能促进血栓形成。2012爱思唯尔爱尔兰有限公司版权所有。
To examine the presence of CD34-positive cells and intranuclear factors in acute coronary thrombi, we compared thrombi in patients with type 2 diabetes mellitus (DM, n = 21) and without DM (n = 29). Immunohistochemical staining revealed the constitutive presence of platelets, fibrin, erythrocytes, neutrophils, extracellular high-mobility group box 1 (HMGB-1), and histone H3 in all thrombi. There were significantly more oval or flat CD34-positive cells and significantly larger HMGB-1-positive areas in the thrombi from patients with DM. The flat CD34-positive cells expressed ecto-nucleoside triphosphate diphosphohydrolase (a platelet aggregation inhibitor). The number of CD34-positive cells was negatively correlated with the serum levels of glucose and hemoglobin A1c, whereas the HMGB-1-positive area was positively correlated with the levels of serum glucose. The paucity of CD34-positive cells and the high levels of HMGB-1 expression in acute coronary thrombi from patients with type 2 DM could facilitate thrombus formation. (C) 2012 Elsevier Ireland Ltd. All rights reserved.